Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Baboon Cell (fetal skeletal muscle)
Permeabilization
No
Specification
fetal skeletal muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal Goat serum in PBS
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated anti-Rabbit Ig G (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
before the primary antibody incubation, sample was treated with Image -iT FX signal enhancer for 30 min. After the secondary antibody incubation, sample was incubated with Streptavidin Alex Fluor 488 (1:500 dilution) in 5% normal Goat serum solution for 30 min in dark , followed by incubation with 1% Sudan Black in 70% MeOH for 10 min and then with propidium iodide (0.1 ug/mL) in PBS for 1 min.

Dr. Soon-Ok Kim

Verified customer

Submitted Oct 02 2017

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