Recombinant Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free
See all Glucose Transporter GLUT4 primary antibodies -
Description
Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glucose Transporter GLUT4 aa 450 to the C-terminus (phospho S488). The exact sequence is proprietary.
Database link: P14672 -
General notes
Ab250949 is the carrier-free version of ab188317. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250949 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
EPR930(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab250949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa). |
Target
-
Function
Insulin-regulated facilitative glucose transporter. -
Tissue specificity
Skeletal and cardiac muscles; brown and white fat. -
Involvement in disease
Diabetes mellitus, non-insulin-dependent -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
-
Database links
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 380691 Human
see all -
Alternative names
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
Images
-
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
All lanes : Human fetal heart whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab188317, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)This data was developed using ab188317, the same antibody clone in a different buffer formulation.Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
-
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab188317, the same antibody clone in a different buffer formulation.
-
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution
Lane 1 : Untreated human fetal heart
Lane 2 : Lambda phosphorylase treated human fetal heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab188317, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab250949 has not yet been referenced specifically in any publications.