RecombinantRabMAb

Recombinant Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)

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Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free
    See all Glucose Transporter GLUT4 primary antibodies

  • Description

    Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT4 aa 450 to the C-terminus (phospho S488). The exact sequence is proprietary.
    Database link: P14672

  • General notes

    Ab250949 is the carrier-free version of ab188317. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250949 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab250949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).

Target

  • Function

    Insulin-regulated facilitative glucose transporter.

  • Tissue specificity

    Skeletal and cardiac muscles; brown and white fat.

  • Involvement in disease

    Diabetes mellitus, non-insulin-dependent

  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

  • Post-translational
    modifications

    Sumoylated.

  • Cellular localization

    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Images

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

    All lanes : Human fetal heart whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    This data was developed using ab188317, the same antibody clone in a different buffer formulation.Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution

    Lane 1 : Untreated human fetal heart
    Lane 2 : Lambda phosphorylase treated human fetal heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab188317, the same antibody clone in a different buffer formulation.

     

  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250949? Please let us know so that we can cite the reference in this datasheet.

ab250949 has not yet been referenced specifically in any publications.

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