Glucose uptake assay (Fluorometric, Direct Glucose) (ab234043)

Overview

  • Product name

    Glucose uptake assay (Fluorometric, Direct Glucose)
    See all Glucose uptake kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Glucose uptake assay (Fluorometric, Direct Glucose) (ab234043) follows a simple and ultra-sensitive protocol to detect glucose uptake in various cells.


    A specific hexokinase inhibitor that inhibits hexokinase, the first enzyme metabolizing glucose in cells is used to arrest glucose consumption after its uptake. Glucose Uptake is measured by using a set of enzymatic reactions that specifically oxidize glucose producing intermediates that react with the Glucose Red Probe generating a fluorescence signal (Ex/Em=535/587 nm). The fluorescence signal is directly proportional to the amount of glucose that has been taken up and accumulated inside the cells. Unlike other kits detecting glucose derivatives, this glucose uptake assay provides a direct, powerful tool for studying this process as well as for screening and characterization of drugs that regulate glucose uptake during normal and disease development.

  • Notes

    Glucose uptake is one of the key processes for cellular glucose metabolism. The study of glucose uptake can provide important information for understanding glucose metabolism and regulation in normal and disease development such as diabetes

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    Assay Buffer 1 x 25ml
    Enzyme Mix 1 vial
    Glucose (1 M, Sterile) 1 x 1ml
    Glucose Red Probe 1 x 200µl
    Glucose Standard (100 mM) 1 x 100µl
    Hexokinase Inhibitor 1 vial

Images

  • Glucose standard curve. 

    Data provided for demonstration purposes only.

  • Jurkat (Human T cell leukemia cell line from peripheral blood) cells were starved (glucose-free, FBS-free medium). Inhibitor incubation time, 2 hours.

  • NIH/3T3 (Mouse embryo fibroblast cell line) cells were starved (glucose-free and FBS-free) for 24 hours then switched to glucose-containing medium and treated +/- insulin (10 ng/ ml) for 15 minutes in the presence or absence of Hexokinase Inhibitor.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were starved for 2 hours (glucose-free and FBS-free media) then switched to either glucose- and FBS-free medium or complete (with 10% FBS) medium with or without Hexokinase Inhibitor for 30 minutes.

Protocols

References

ab234043 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234043.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up