Overview

  • Product name

    Glucose Uptake Assay Kit (Cell-based)
    See all Glucose kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Glucose Uptake Assay Kit (Cell-based) (ab204702) is an easy-to-use, non-radioactive kit that allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin and growth factors.


    The glucose uptake assay protocol is based on a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by the fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry.


    The kit includes phloretin, a natural phenol that inhibits glucose uptake, to use with control samples.


    Glucose uptake assay protocol summary:
    - remove cell culture media, and add glucose uptake mix to cells
    - incubate for 30 min at 37ºC
    - collect cells and keep on ice
    - wash with analysis buffer
    - analyze with flow cytometer or fluorescent microscope

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    Analysis Buffer (50X) 1 x 1.8ml
    GluTracker Enhancer 1 x 1ml
    GluTracker Reagent (100X) 1 x 200µl
    Phloretin (100X) 1 x 75µl
  • Research areas

  • Relevance

    Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.

Images

  • Comparison of histogram from flow analysis showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin).

  • Images of Jurkat cells obtained using fluorescent microscope (Top: treated with phloretin; Bottom: without phloretin treatment).

  • Glucose Uptake in HeLa cells: HeLa cells showing the uptake of GluTracker Reagent in the cytoplasm. Cells were stained with GluTracker Reagent for 30 minutes and fixed. Image was taken using a fluorescent microscope with a 60X objective lens.

Protocols

References

This product has been referenced in:

  • Valentín-Guillama G  et al. HIV-1 Envelope Protein gp120 Promotes Proliferation and the Activation of Glycolysis in Glioma Cell. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30200472) »
See 1 Publication for this product

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