Glucose Uptake Assay Kit (Colorimetric) (ab136955)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 0.01 nmol/well
Overview
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Product name
Glucose Uptake Assay Kit (Colorimetric)
See all Glucose uptake kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Sensitivity
<= 0.01 nmol/well -
Assay time
3h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glucose Uptake Assay Kit (Colorimetric) (ab136955) is a highly sensitive and easy to use non-radioactive assay kit which can detect glucose uptake as low as 10 pmol/well in a variety of cell types.
2-deoxyglucose (2-DG) is used in glucose uptake assay protocols because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the 2-DG6P is oxidized to generate NADPH, the level of which can be determined by an enzymatic recycling amplification reaction.
Glucose uptake assay protocol summary:
- prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up
- add 2-DG to cells and incubate for 20 mins at 37ºC
- wash cells with PBS to remove exogenous 2-DG
- lyse cells with extraction buffer and repeated pipetting
- freeze/thaw lysates and heat at 85ºC for 40 min
- cool on ice for 5 min
- add neutralizing buffer, spin and transfer supernatant to new tubes
- add supernatants and standards to wells
- add reaction mix A and incubate for 1 hr at 37ºC
- add extraction buffer and heat to 90ºC for 40 min
- cool on ice for 5 min and add neutralizing buffer
- add reaction mix B
- analyze every 2-3 mins on microplate reader in kinetic mode at 37ºC
TEST -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K676 Glucose Uptake Colorimetric Assay Kit. K676-100 is the same size as the 100 test size of ab136955.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2-Deoxyglucose 1 x 1ml 2-DG6P Standard 1 vial Assay Buffer III 1 x 25ml DTNB 2 vials Enzyme Mix III 1 vial Extraction Buffer I 1 x 17ml Glutathione Reductase 2 vials Neutralization Buffer II 1 x 2.5ml Recycling Mix 1 vial -
Research areas
Associated products
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Related Products
Images
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2-DG6P Standard curve (a) and 2-DG uptake in 3T3-L1 cells (b), Human adipocytes (c) and HeLa cells (d) respectively. I=Insulin; P=Phloretin.
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Step A: 2-DG oxidation to generate NADPH; Step B: NADPH recycling amplification Reaction.
Datasheets and documents
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SDS download
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Datasheet download
References (159)
ab136955 has been referenced in 159 publications.
- Sun X et al. Glucose transporter GLUT1 expression is important for oriental river prawn (Macrobrachium nipponense) hemocyte adaptation to hypoxic conditions. J Biol Chem 299:102748 (2023). PubMed: 36436564
- Wang Q et al. YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Exp Ther Med 25:37 (2023). PubMed: 36569438
- Ding L et al. Canagliflozin primes antitumor immunity by triggering PD-L1 degradation in endocytic recycling. J Clin Invest 133:N/A (2023). PubMed: 36594471
- Wang YJ et al. Never-in-Mitosis A-Related Kinase 8 (NEK8) Regulates Adipogenesis, Glucose Homeostasis, and Obesity. Oxid Med Cell Longev 2022:1947067 (2022). PubMed: 36506932
- Li X et al. Long non-coding RNA CTSLP8 mediates ovarian cancer progression and chemotherapy resistance by modulating cellular glycolysis and regulating c-Myc expression through PKM2. Cell Biol Toxicol 38:1027-1045 (2022). PubMed: 34510316