Recombinant
RabMAb

Recombinant Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071)

Overview

  • Product name

    Anti-Glucosidase 2 subunit beta antibody [EPR8046]
    See all Glucosidase 2 subunit beta primary antibodies
  • Description

    Rabbit monoclonal [EPR8046] to Glucosidase 2 subunit beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glucosidase 2 subunit beta aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat and A431 cell lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Regulatory subunit of glucosidase II.
    • Pathway

      Glycan metabolism; N-glycan metabolism.
    • Involvement in disease

      Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.
    • Sequence similarities

      Contains 2 EF-hand domains.
      Contains 1 PRKCSH domain.
    • Cellular localization

      Endoplasmic reticulum.
    • Information by UniProt
    • Database links

    • Alternative names

      • 80K-H protein antibody
      • AGE-binding receptor 2 antibody
      • AGE-R2 antibody
      • G19P1 antibody
      • GLU2B_HUMAN antibody
      • Glucosidase 2 subunit beta antibody
      • Glucosidase II beta subunit antibody
      • Glucosidase II subunit beta antibody
      • Hepatocystin antibody
      • PCLD antibody
      • PKCSH antibody
      • PLD1 antibody
      • PRKCSH antibody
      • Protein kinase C substrate 60.1 kDa protein heavy chain antibody
      • Protein kinase C substrate 80 Kda protein antibody
      • Protein kinase C substrate 80K-H antibody
      • Protein kinase C substrate, 80 Kda protein antibody
      see all

    Images

    • All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Glucosidase 2 subunit beta with ab134071 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab134071 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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