Recombinant Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8046] to Glucosidase 2 subunit beta
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucosidase 2 subunit beta antibody [EPR8046]
See all Glucosidase 2 subunit beta primary antibodies -
Description
Rabbit monoclonal [EPR8046] to Glucosidase 2 subunit beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Glucosidase 2 subunit beta aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HEK293T, Daudi, HeLa, Jurkat and A431 cell lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8046 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Regulatory subunit of glucosidase II. -
Pathway
Glycan metabolism; N-glycan metabolism. -
Involvement in disease
Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 PRKCSH domain. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 5589 Human
- Entrez Gene: 19089 Mouse
- Entrez Gene: 300445 Rat
- Omim: 177060 Human
- SwissProt: P14314 Human
- SwissProt: O08795 Mouse
- Unigene: 610830 Human
- Unigene: 214593 Mouse
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Alternative names
- 80K-H protein antibody
- AGE-binding receptor 2 antibody
- AGE-R2 antibody
see all
Images
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All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PRKCSH knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1- 4: Merged signal (red and green). Green - ab134071 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab134071 was shown to react with PRKCSH in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266770 (knockout cell lysate ab257608) was used. Wild-type HEK-293T and PRKCSH knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134071 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Glucosidase 2 subunit beta with ab134071 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134071 has been referenced in 2 publications.
- Droujinine IA et al. Proteomics of protein trafficking by in vivo tissue-specific labeling. Nat Commun 12:2382 (2021). PubMed: 33888706
- Wächter K et al. AGE-Rich Bread Crust Extract Boosts Oxidative Stress Interception via Stimulation of the NRF2 Pathway. Nutrients 13:N/A (2021). PubMed: 34836129