Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8046] to Glucosidase 2 subunit beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Glucosidase 2 subunit beta antibody [EPR8046]
See all Glucosidase 2 subunit beta primary antibodies
DescriptionRabbit monoclonal [EPR8046] to Glucosidase 2 subunit beta
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Glucosidase 2 subunit beta aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa, Jurkat and A431 cell lysates; Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionRegulatory subunit of glucosidase II.
PathwayGlycan metabolism; N-glycan metabolism.
Involvement in diseaseDefects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.
Sequence similaritiesContains 2 EF-hand domains.
Contains 1 PRKCSH domain.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- 80K-H protein antibody
- AGE-binding receptor 2 antibody
- AGE-R2 antibody
All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8046] (ab134071) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Glucosidase 2 subunit beta with ab134071 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab134071 has not yet been referenced specifically in any publications.