Recombinant
RabMAb

Recombinant Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098)

Overview

  • Product name

    Anti-Glucosidase 2 subunit beta antibody [EPR8047]
    See all Glucosidase 2 subunit beta primary antibodies
  • Description

    Rabbit monoclonal [EPR8047] to Glucosidase 2 subunit beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glucosidase 2 subunit beta aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, A431, and K562 cell lysates; Human brain tissue; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 59 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.

Target

  • Function

    Regulatory subunit of glucosidase II.
  • Pathway

    Glycan metabolism; N-glycan metabolism.
  • Involvement in disease

    Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.
  • Sequence similarities

    Contains 2 EF-hand domains.
    Contains 1 PRKCSH domain.
  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • 80K-H protein antibody
    • AGE-binding receptor 2 antibody
    • AGE-R2 antibody
    • G19P1 antibody
    • GLU2B_HUMAN antibody
    • Glucosidase 2 subunit beta antibody
    • Glucosidase II beta subunit antibody
    • Glucosidase II subunit beta antibody
    • Hepatocystin antibody
    • PCLD antibody
    • PKCSH antibody
    • PLD1 antibody
    • PRKCSH antibody
    • Protein kinase C substrate 60.1 kDa protein heavy chain antibody
    • Protein kinase C substrate 80 Kda protein antibody
    • Protein kinase C substrate 80K-H antibody
    • Protein kinase C substrate, 80 Kda protein antibody
    see all

Images

  • ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : A431 cell lysates
    Lane 4 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa

  • ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in HeLa cells by Immunofluorescence.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

ab129098 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129098.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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