Anti-Glucuronic Acid Epimerase/GLCE antibody (ab68714)
Key features and details
- Mouse polyclonal to Glucuronic Acid Epimerase/GLCE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucuronic Acid Epimerase/GLCE antibody
See all Glucuronic Acid Epimerase/GLCE primary antibodies -
Description
Mouse polyclonal to Glucuronic Acid Epimerase/GLCE -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Glucuronic Acid Epimerase/GLCE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_056369.1 -
Positive control
- Glucuronic Acid Epimerase/GLCE transfected 293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
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Relevance
Glucuronic Acid Epimerase (GLCE) is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of Heparan sulfate (HS), which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]). -
Cellular localization
Golgi apparatus membrane; Single-pass type II membrane protein -
Database links
- Entrez Gene: 26035 Human
- Omim: 612134 Human
- SwissProt: O94923 Human
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Alternative names
- D glucuronyl C5 epimerase antibody
- GLCE antibody
- Heparin/heparan sulfate:glucuronic acid C5 epimerase antibody
see all
Images
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All lanes : Anti-Glucuronic Acid Epimerase/GLCE antibody (ab68714) at 1/500 dilution
Lane 1 : Glucuronic Acid Epimerase/GLCE transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa
Datasheets and documents
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Datasheet download
References (1)
ab68714 has been referenced in 1 publication.
- Small EM et al. MicroRNA-218 regulates vascular patterning by modulation of Slit-Robo signaling. Circ Res 107:1336-44 (2010). WB ; Human, Mouse . PubMed: 20947829