RecombinantRabMAb

Recombinant Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

Reviews (2) Submit a question References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
  • Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GLUD1 + GLUD2 antibody [EPR11369(B)]

  • Description

    Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2

  • Host species

    Rabbit

  • Specificity

    Ab166618 immunogen shows 100% identity to human GLUD2.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
    Database link: P00367

  • Positive control

    • Human fetal liver, HepG2, HeLa and 293T lysates, Human liver & skeletal muscle tissue, permeabilized 293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab166618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal tonsil.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : HeLa lysates
      Lane 4 : 293T lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal colon.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal brain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
      Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)

    References (8)

    Publishing research using ab166618? Please let us know so that we can cite the reference in this datasheet.

    ab166618 has been referenced in 8 publications.

    • Lin Y  et al. Canagliflozin impairs blood reperfusion of ischaemic lower limb partially by inhibiting the retention and paracrine function of bone marrow derived mesenchymal stem cells. EBioMedicine 52:102637 (2020). PubMed: 31981975
    • Fu S  et al. Glutamine Synthetase Promotes Radiation Resistance via Facilitating Nucleotide Metabolism and Subsequent DNA Damage Repair. Cell Rep 28:1136-1143.e4 (2019). PubMed: 31365859
    • Sukovas A  et al. Hyperthermia potentiates cisplatin cytotoxicity and negative effects on mitochondrial functions in OVCAR-3 cells. J Bioenerg Biomembr 51:301-310 (2019). PubMed: 31332716
    • Duan G  et al. Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth. Sci Rep 8:4067 (2018). PubMed: 29511244
    • Roux C  et al. Endogenous glutamine decrease is associated with pancreatic cancer progression. Oncotarget 8:95361-95376 (2017). PubMed: 29221133
    • Meyer KA  et al. Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer. Biochem Biophys Rep 7:144-149 (2016). WB ; Mouse . PubMed: 27617308
    • Miyo M  et al. Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer. Sci Rep 6:38415 (2016). PubMed: 27924922
    • Chaumeil MM  et al. Hyperpolarized [1-13C] glutamate: a metabolic imaging biomarker of IDH1 mutational status in glioma. Cancer Res 74:4247-57 (2014). PubMed: 24876103

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GLUD1 antibody [EPR11369(B)]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain,neurons and astrocytes)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X-100
    Specification
    brain,neurons and astrocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Mohammad Khamaisy

    Verified customer

    Submitted May 14 2020

    Western blot abreview for Anti-GLUD1 antibody [EPR11369(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HCT116 colon cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 02 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com