Recombinant Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] -
Description
Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2 -
Host species
Rabbit -
Specificity
Ab166618 immunogen shows 100% identity to human GLUD2.
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Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
Database link: P00367 -
Positive control
- IHC-P: Human tonsil tissue; Human colon tissue; Human kidney tissue; Human brain tissue; Human liver tissue; Human skeletal muscle tissue. WB: Human fetal liver lysates, HeLa cell lysates, HepG2 lysates, 293T lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11369(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
GLUD1: Mitochondrion matrix. GLUD2: Mitochondrion matrix. -
Database links
- Entrez Gene: 2746 Human
- Entrez Gene: 2747 Human
- Entrez Gene: 104277 Mouse
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- GenBank: NM_012084.3 Human
- Omim: 138130 Human
- Omim: 300144 Human
see all
Images
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ab166618 showing +ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : HeLa lysates
Lane 4 : 293T lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa -
ab166618 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab166618 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab166618 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab166618 has been referenced in 16 publications.
- Suzuki T et al. Mutant KRAS drives metabolic reprogramming and autophagic flux in premalignant pancreatic cells. Cancer Gene Ther 29:505-518 (2022). PubMed: 33833413
- Kerr M et al. Diabetic mitochondria are resistant to palmitoyl CoA inhibition of respiration, which is detrimental during ischemia. FASEB J 35:e21765 (2021). PubMed: 34318967
- Park MK et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Cell Metab 33:2380-2397.e9 (2021). PubMed: 34879239
- Papadopoli D et al. Perturbations of cancer cell metabolism by the antidiabetic drug canagliflozin. Neoplasia 23:391-399 (2021). PubMed: 33784591
- He Y et al. miR-30a-5p inhibits hypoxia/reoxygenation-induced oxidative stress and apoptosis in HK-2 renal tubular epithelial cells by targeting glutamate dehydrogenase 1 (GLUD1). Oncol Rep 44:1539-1549 (2020). PubMed: 32945480