Recombinant Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (ab249357)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free -
Description
Rabbit monoclonal [EPR11369(B)] to GLUD1 + GLUD2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody immunogen shows 100% identity to human GLUD2.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
Database link: P00367 -
General notes
Ab249357 is the carrier-free version of ab166618. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249357 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11369(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab249357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
Target
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Cellular localization
GLUD1: Mitochondrion matrix. GLUD2: Mitochondrion matrix. -
Database links
- Entrez Gene: 2746 Human
- Entrez Gene: 2747 Human
- Entrez Gene: 104277 Mouse
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- GenBank: NM_012084.3 Human
- Omim: 138130 Human
- Omim: 300144 Human
see all
Images
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All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : HeLa lysates
Lane 4 : 293T lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDaThis data was developed using ab166618, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (ab249357)This data was developed using ab166618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11369(B)] - BSA and Azide free (ab249357)This data was developed using ab166618, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab249357 has not yet been referenced specifically in any publications.