Name: Anti-GLUD1 + GLUD2 antibody [EPR11370]
Brand: RabMAb
SKU: ab168352
Rating: 3 (1 reviews)

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Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-GLUD1 + GLUD2 antibody [EPR11370]
Description

Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2
Host species

Rabbit
Specificity

Ab168352 immunogen shows 93% identity to human GLUD2.
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

within Human GLUD1 aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P00367
Positive control
- WB: Human fetal liver tissue lysate and HepG2 whole cell lysate (ab7900). IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11370
Isotype

IgG
Research areas

Alternative Versions
- Alexa Fluor® 488 Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab204001)
- Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant GLUD1 protein (Active) (ab219093)

Our Abpromise guarantee covers the use of ab168352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/50 - 1/100.
Flow Cyt		1/2000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IP.

Cellular localization

GLUD1: Mitochondrion matrix. GLUD2: Mitochondrion matrix.
Database links
- Entrez Gene: 2746 Human
- Entrez Gene: 2747 Human
- Entrez Gene: 104277 Mouse
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- GenBank: NM_012084.3 Human
- Omim: 138130 Human
- Omim: 300144 Human
- SwissProt: P00367 Human
- SwissProt: P49448 Human
- SwissProt: P26443 Mouse
- SwissProt: P10860 Rat
- Unigene: 368538 Human
- Unigene: 500409 Human
- Unigene: 10600 Mouse
- Unigene: 55106 Rat
see all

Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

ab168352 showing +ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution.
Flow Cytometry - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

ab168352 showing +ve staining in Human normal uterus.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

ab168352 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab168352? Please let us know so that we can cite the reference in this datasheet.

ab168352 has been referenced in 4 publications.

Sun N et al. Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis. Int J Mol Med 44:2189-2200 (2019). PubMed: 31661119
Craze ML et al. Glutamate dehydrogenase (GLUD1) expression in breast cancer. Breast Cancer Res Treat N/A:N/A (2018). PubMed: 30470977
Li W et al. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma. J Cell Mol Med 21:2896-2908 (2017). WB . PubMed: 28544376
Vázquez-Martínez O et al. Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location. Exp Biol Med (Maywood) 242:945-952 (2017). PubMed: 28440738

Customer reviews and Q&As

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Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Brain, Hippocampus)

Antigen retrieval step

None

Permeabilization

Yes - Triton x100

Specification

Brain, Hippocampus

Blocking step

Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

Mr. Mohammad Khamaisy

Verified customer

Submitted Jun 07 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

Target

Cellular localization

Database links

Images

Protocols

Datasheets and documents

References (4)

Customer reviews and Q&As

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GLUD1 antibody [EPR11370]