Name: Anti-GLUD1 antibody [EPR11369(B)]
Brand: RabMAb
SKU: ab166618
Rating: 5 (1 reviews)

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Primary

Protein

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Primary - Rabbit Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
WB, IHC-P, IP

Protein - Recombinant GLUD1 protein (Active) (ab219093)
Functional Studies

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Product name

Anti-GLUD1 antibody [EPR11369(B)]
See all GLUD1 primary antibodies
Description

Rabbit monoclonal [EPR11369(B)] to GLUD1
Host species

Rabbit
Specificity

Ab166618 immunogen shows 100% identity to human GLUD2, therefore there exists a potential for cross-reactivity.
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
Database link: P00367
Positive control
- Human fetal liver, HepG2, HeLa and 293T lysates, Human liver & skeletal muscle tissue, permeabilized 293T cells
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11369(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-GLUD1 antibody [EPR11369(B)] - BSA and Azide free (ab249357)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant GLUD1 protein (Active) (ab219093)

Our Abpromise guarantee covers the use of ab166618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P		1/250 - 1/500.
IP		1/10 - 1/100.

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Involvement in disease

Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Cellular localization

Mitochondrion matrix.
Target information above from: UniProt accession P00367 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2746 Human
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- Omim: 138130 Human
- SwissProt: P00367 Human
- SwissProt: P26443 Mouse
- SwissProt: P10860 Rat
- Unigene: 500409 Human
- Unigene: 10600 Mouse
- Unigene: 55106 Rat
see all
Alternative names
- AI118167 antibody
- DHE3_HUMAN antibody
- EC 1.4.1.3 antibody
- GDH 1 antibody
- GDH antibody
- Gdh-X antibody
- GDH1 antibody
- GLUD antibody
- Glud1 antibody
- Glud1a antibody
- Glud1b antibody
- Gludl antibody
- Glutamate dehydrogenase (NAD(P)+) antibody
- Glutamate dehydrogenase 1 antibody
- Glutamate dehydrogenase 1 mitochondrial antibody
- Glutamate dehydrogenase 1a antibody
- Glutamate dehydrogenase 1b antibody
- Memory-related gene 2 protein antibody
- MGC127177 antibody
- MGC132003 antibody
- MGC80801 antibody
- MGC93608 antibody
- mitochondrial antibody
- MRG-2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

ab166618 showing +ve staining in Human normal tonsil.
Western blot - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

All lanes : Anti-GLUD1 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution

Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : HeLa lysates
Lane 4 : 293T lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

ab166618 showing +ve staining in Human normal colon.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

ab166618 showing +ve staining in Human normal kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

ab166618 showing +ve staining in Human normal brain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Fu S et al. Glutamine Synthetase Promotes Radiation Resistance via Facilitating Nucleotide Metabolism and Subsequent DNA Damage Repair. Cell Rep 28:1136-1143.e4 (2019). Read more (PubMed: 31365859) »
Sukovas A et al. Hyperthermia potentiates cisplatin cytotoxicity and negative effects on mitochondrial functions in OVCAR-3 cells. J Bioenerg Biomembr 51:301-310 (2019). Read more (PubMed: 31332716) »

See all 7 Publications for this product

Publishing research using ab166618? Please let us know so that we can cite the reference in this datasheet.

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Application

Western blot

Sample

Human Cell lysate - whole cell (HCT116 colon cancer cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

HCT116 colon cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 02 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-GLUD1 antibody [EPR11369(B)]