Recombinant
RabMAb

Recombinant Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11369(B)] to GLUD1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GLUD1 antibody [EPR11369(B)]
    See all GLUD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11369(B)] to GLUD1

  • Host species

    Rabbit

  • Specificity

    Ab166618 immunogen shows 100% identity to human GLUD2, therefore there exists a potential for cross-reactivity.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
    Database link: P00367

  • Positive control

    • Human fetal liver, HepG2, HeLa and 293T lysates, Human liver & skeletal muscle tissue, permeabilized 293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab166618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.

    • Involvement in disease

      Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.

    • Sequence similarities

      Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AI118167 antibody
      • DHE3_HUMAN antibody
      • EC 1.4.1.3 antibody
      • GDH 1 antibody
      • GDH antibody
      • Gdh-X antibody
      • GDH1 antibody
      • GLUD antibody
      • Glud1 antibody
      • Glud1a antibody
      • Glud1b antibody
      • Gludl antibody
      • Glutamate dehydrogenase (NAD(P)+) antibody
      • Glutamate dehydrogenase 1 antibody
      • Glutamate dehydrogenase 1 mitochondrial antibody
      • Glutamate dehydrogenase 1a antibody
      • Glutamate dehydrogenase 1b antibody
      • Memory-related gene 2 protein antibody
      • MGC127177 antibody
      • MGC132003 antibody
      • MGC80801 antibody
      • MGC93608 antibody
      • mitochondrial antibody
      • MRG-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal tonsil.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Western blot - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      All lanes : Anti-GLUD1 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : HeLa lysates
      Lane 4 : 293T lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal colon.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      ab166618 showing +ve staining in Human normal brain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    Publishing research using ab166618? Please let us know so that we can cite the reference in this datasheet.

    ab166618 has been referenced in 8 publications.

    • Lin Y  et al. Canagliflozin impairs blood reperfusion of ischaemic lower limb partially by inhibiting the retention and paracrine function of bone marrow derived mesenchymal stem cells. EBioMedicine 52:102637 (2020). PubMed: 31981975
    • Fu S  et al. Glutamine Synthetase Promotes Radiation Resistance via Facilitating Nucleotide Metabolism and Subsequent DNA Damage Repair. Cell Rep 28:1136-1143.e4 (2019). PubMed: 31365859
    • Sukovas A  et al. Hyperthermia potentiates cisplatin cytotoxicity and negative effects on mitochondrial functions in OVCAR-3 cells. J Bioenerg Biomembr 51:301-310 (2019). PubMed: 31332716
    • Duan G  et al. Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth. Sci Rep 8:4067 (2018). PubMed: 29511244
    • Roux C  et al. Endogenous glutamine decrease is associated with pancreatic cancer progression. Oncotarget 8:95361-95376 (2017). PubMed: 29221133
    • Meyer KA  et al. Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer. Biochem Biophys Rep 7:144-149 (2016). WB ; Mouse . PubMed: 27617308
    • Miyo M  et al. Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer. Sci Rep 6:38415 (2016). PubMed: 27924922
    • Chaumeil MM  et al. Hyperpolarized [1-13C] glutamate: a metabolic imaging biomarker of IDH1 mutational status in glioma. Cancer Res 74:4247-57 (2014). PubMed: 24876103

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GLUD1 antibody [EPR11369(B)]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain,neurons and astrocytes)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X-100
    Specification
    brain,neurons and astrocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mr. Mohammad Khamaisy

    Verified customer

    Submitted May 14 2020

    Western blot abreview for Anti-GLUD1 antibody [EPR11369(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HCT116 colon cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 02 2018

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