Recombinant
Anti-GLUD1 antibody [EPR11369(B)] (ab166618)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-GLUD1 antibody [EPR11369(B)]
See all GLUD1 primary antibodies -
DescriptionRabbit monoclonal [EPR11369(B)] to GLUD1
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Host speciesRabbit
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SpecificityAb166618 immunogen shows 100% identity to human GLUD2, therefore there exists a potential for cross-reactivity.
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Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human GLUD1 aa 50-150. The exact sequence is proprietary.
Database link: P00367 -
Positive control
- Human fetal liver, HepG2, HeLa and 293T lysates, Human liver & skeletal muscle tissue, permeabilized 293T cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11369(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab166618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. | |
| IHC-P | 1/250 - 1/500. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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FunctionMay be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
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Involvement in diseaseDefects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
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Sequence similaritiesBelongs to the Glu/Leu/Phe/Val dehydrogenases family.
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Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 2746 Human
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- Omim: 138130 Human
- SwissProt: P00367 Human
- SwissProt: P26443 Mouse
- SwissProt: P10860 Rat
- Unigene: 500409 Human
see all -
Alternative names
- AI118167 antibody
- DHE3_HUMAN antibody
- EC 1.4.1.3 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/ab166618-8-YCAR11369-glutamate-dehydr-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)ab166618 showing +ve staining in Human normal tonsil. -
![Western blot - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/Glutamate-Dehydrogenase-Primary-antibodies-ab166618-2.jpg)
Western blot - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)All lanes : Anti-GLUD1 antibody [EPR11369(B)] (ab166618) at 1/10000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : HeLa lysates
Lane 4 : 293T lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/ab166618-7-YCAR11369-glutamate-dehydr-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)ab166618 showing +ve staining in Human normal colon. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/ab166618-6-YCAR11369-glutamat-dehydr-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)ab166618 showing +ve staining in Human normal kidney. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/ab166618-5-YCAR11369-glutamat-dehydr-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)ab166618 showing +ve staining in Human normal brain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/Glutamate-Dehydrogenase-Primary-antibodies-ab166618-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling GLUD1 with ab166618 at 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)](https://www.abcam.com/ps/products/166/ab166618/Images/Glutamate-Dehydrogenase-Primary-antibodies-ab166618-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 antibody [EPR11369(B)] (ab166618)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GLUD1 with ab166618 at 1/250 dilution.
Datasheets and documents
References
This product has been referenced in:
- Duan G et al. Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth. Sci Rep 8:4067 (2018). Read more (PubMed: 29511244) »
- Roux C et al. Endogenous glutamine decrease is associated with pancreatic cancer progression. Oncotarget 8:95361-95376 (2017). Read more (PubMed: 29221133) »
