Recombinant
RabMAb

Recombinant Anti-GLUD1 antibody [EPR11370] (Alexa Fluor® 488) (ab204001)

Overview

  • Product name

    Anti-GLUD1 antibody [EPR11370] (Alexa Fluor® 488)
    See all GLUD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11370] to GLUD1 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GLUD1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P00367

  • Positive control

    • ICC/IF: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab204001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Function

    May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
  • Involvement in disease

    Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
  • Sequence similarities

    Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI118167 antibody
    • DHE3_HUMAN antibody
    • EC 1.4.1.3 antibody
    • GDH 1 antibody
    • GDH antibody
    • Gdh-X antibody
    • GDH1 antibody
    • GLUD antibody
    • Glud1 antibody
    • Glud1a antibody
    • Glud1b antibody
    • Gludl antibody
    • Glutamate dehydrogenase (NAD(P)+) antibody
    • Glutamate dehydrogenase 1 antibody
    • Glutamate dehydrogenase 1 mitochondrial antibody
    • Glutamate dehydrogenase 1a antibody
    • Glutamate dehydrogenase 1b antibody
    • Memory-related gene 2 protein antibody
    • MGC127177 antibody
    • MGC132003 antibody
    • MGC80801 antibody
    • MGC93608 antibody
    • mitochondrial antibody
    • MRG-2 antibody
    see all

Images

  • ab204001 staining GLUD1 in HepG2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204001 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).

References

ab204001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204001.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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