Recombinant
RabMAb

Recombinant Anti-GLUD1 antibody [EPR11370] - BSA and Azide free (ab228340)

Overview

  • Product name

    Anti-GLUD1 antibody [EPR11370] - BSA and Azide free
    See all GLUD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11370] to GLUD1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody immunogen shows 93% identity to human GLUD2, therefore there exists a potential for cross-reactivity.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GLUD1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P00367

  • Positive control

    • WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
  • General notes

    Ab228340 is the carrier-free version of ab168352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
    • Involvement in disease

      Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
    • Sequence similarities

      Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI118167 antibody
      • DHE3_HUMAN antibody
      • EC 1.4.1.3 antibody
      • GDH 1 antibody
      • GDH antibody
      • Gdh-X antibody
      • GDH1 antibody
      • GLUD antibody
      • Glud1 antibody
      • Glud1a antibody
      • Glud1b antibody
      • Gludl antibody
      • Glutamate dehydrogenase (NAD(P)+) antibody
      • Glutamate dehydrogenase 1 antibody
      • Glutamate dehydrogenase 1 mitochondrial antibody
      • Glutamate dehydrogenase 1a antibody
      • Glutamate dehydrogenase 1b antibody
      • Memory-related gene 2 protein antibody
      • MGC127177 antibody
      • MGC132003 antibody
      • MGC80801 antibody
      • MGC93608 antibody
      • mitochondrial antibody
      • MRG-2 antibody
      see all

    References

    ab228340 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab228340.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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