Overview

  • Product name

    Anti-GLUD1 + GLUD2 antibody
    See all GLUD1 + GLUD2 primary antibodies
  • Description

    Rabbit polyclonal to GLUD1 + GLUD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GLUD1 + GLUD2 aa 294-558. Recombinant protein encompassing a sequence within the center region of human GLUD1+2. GLUD1: SwissProt ID: P00367.
    Database link: P49448

  • Positive control

    • 293T, A431, HeLa, HepG2 and Mouse testis whole cell lysates; HeLa cells; Human Hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    GLUD1: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. GLUD2: Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
  • Database links

  • Alternative names

    • GDH 1 antibody
    • GDH 2 antibody
    • GLUD antibody
    • GLUD1 antibody
    • GLUD2 antibody
    • GLUDP1 antibody
    • Glutamate dehydrogenase 1, mitochondrial antibody
    • Glutamate dehydrogenase 2, mitochondrial antibody
    see all

Images

  • Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution + Mouse testis whole cell lysate at 50 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • All lanes : Anti-GLUD1 + GLUD2 antibody (ab154027) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Hepatoma labeling GLUD1 + GLUD2 with ab154027 at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling GLUD1 + GLUD2 with ab154027 antibody at 1/500 dilution. Bottom figure: cells costained with Hoechst 33342.

References

ab154027 has not yet been referenced specifically in any publications.

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