Product nameAnti-GLUD1 + GLUD2 antibody
See all GLUD1 + GLUD2 primary antibodies
DescriptionGoat polyclonal to GLUD1 + GLUD2
Specificityab99470 is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Chimpanzee
- WB: Human, mouse, and rat brain and human, mouse and rat liver tissue lysates. IHC-P: Human cerebral cortex tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99470 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab99470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 61 kDa.|
RelevanceGLUD1: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. GLUD2: Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
- GDH 1 antibody
- GDH 2 antibody
- GLUD antibody
All lanes : Anti-GLUD1 + GLUD2 antibody (ab99470) at 0.03 µg/ml
Lane 1 : Human brain tissue lysate in RIPA buffer
Lane 2 : Mouse brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation for 1 hour. Detected using chemiluminescence.
ab99470 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex hows mitochondrial staining in neuronal cell bodies and axons. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer) labelling GLUD1 + GLUD2 with ab99470 at a concentration of 0.01µg/ml for 1 hour.
This product has been referenced in:
- Kaneko Y et al. Extracellular HMGB1 Modulates Glutamate Metabolism Associated with Kainic Acid-Induced Epilepsy-Like Hyperactivity in Primary Rat Neural Cells. Cell Physiol Biochem 41:947-959 (2017). Read more (PubMed: 28222432) »