Anti-GluK1 antibody (ab118891)
Key features and details
- Rabbit polyclonal to GluK1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-GluK1 antibody
See all GluK1 primary antibodies -
Description
Rabbit polyclonal to GluK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
-
Immunogen
Synthetic peptide corresponding to Human GluK1.
-
General notes
This product was previously labelled as GRIK1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab118891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 104 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. -
Tissue specificity
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2897 Human
- Entrez Gene: 14805 Mouse
- Entrez Gene: 29559 Rat
- Omim: 138245 Human
- SwissProt: P39086 Human
- SwissProt: Q60934 Mouse
- SwissProt: P22756 Rat
- Unigene: 664641 Human
see all -
Alternative names
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (ab118891)
ab118891 at 5 µg/ml staining GluK1 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Western blot - Anti-GluK1 antibody (ab118891)Lane 1 : Anti-GluK1 antibody (ab118891) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab118891) at 1 µg/ml
All lanes : Rat Brain Tissue Lysate
Predicted band size: 104 kDa
Protocols
Datasheets and documents
References (1)
ab118891 has been referenced in 1 publication.
- Perez-Ortiz AC et al. Pharmacogenetics of response to neoadjuvant paclitaxel treatment for locally advanced breast cancer. Oncotarget 8:106454-106467 (2017). PubMed: 29290962
