Anti-GluK1 antibody (ab118891)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-GluK1 antibody
See all GluK1 primary antibodies -
Description
Rabbit polyclonal to GluK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human GluK1.
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Positive control
- Human Adrenal tissue; Rat brain tissue lysate.
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General notes
This product was previously labelled as GRIK1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab118891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 104 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. -
Tissue specificity
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2897 Human
- Entrez Gene: 14805 Mouse
- Entrez Gene: 29559 Rat
- Omim: 138245 Human
- SwissProt: P39086 Human
- SwissProt: Q60934 Mouse
- SwissProt: P22756 Rat
- Unigene: 664641 Human
see all -
Alternative names
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK1 antibody (ab118891)ab118891 at 5 µg/ml staining GluK1 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-GluK1 antibody (ab118891)Lane 1 : Anti-GluK1 antibody (ab118891) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab118891) at 1 µg/ml
All lanes : Rat Brain Tissue Lysate
Predicted band size: 104 kDa
Datasheets and documents
References
This product has been referenced in:
- Perez-Ortiz AC et al. Pharmacogenetics of response to neoadjuvant paclitaxel treatment for locally advanced breast cancer. Oncotarget 8:106454-106467 (2017). Read more (PubMed: 29290962) »
