Key features and details
- Rabbit polyclonal to GluK1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GluK1 antibody
See all GluK1 primary antibodies
DescriptionRabbit polyclonal to GluK1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human GluK1 (C terminal).
- P815 cells. Brain tissue.
This product was previously labelled as GRIK1
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).|
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus.
Tissue specificityMost abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EAA3 antibody
- EEA3 antibody
- Excitatory amino acid receptor 3 antibody
Immunofluorescence of GluK1 in Mouse Brain cells using ab67316 at 20 ug/ml.
Paraffin-embedded mouse brain tissue stained for GluK1 using ab67316 at 5 µg/ml in immunohistochemical analysis.
Lane 1 : Anti-GluK1 antibody (ab67316) at 0.5 µg/ml
Lane 2 : Anti-GluK1 antibody (ab67316) at 1 µg/ml
Lane 3 : Anti-GluK1 antibody (ab67316) at 2 µg/ml
All lanes : P815 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence of GluK1 in Human Brain cells using ab67316 at 20 ug/ml.
ab67316 has not yet been referenced specifically in any publications.