Product nameAnti-GluK5 antibody
See all GluK5 primary antibodies
DescriptionRabbit polyclonal to GluK5
Specificityab67408 does not cross-react with GRIK4.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human GluK5 (C terminal).
- Human brain tissue and brain tissue lysate.
This product was previously labelled as GRIK5
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 109 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EAA 2 antibody
- EAA2 antibody
- Excitatory amino acid receptor 2 antibody
Lane 1 : Anti-GluK5 antibody (ab67408) at 0.5 µg/ml
Lane 2 : Anti-GluK5 antibody (ab67408) at 1 µg/ml
Lane 3 : Anti-GluK5 antibody (ab67408) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 109 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
ab67408 at 2.5µg/ml staining of GluK5 in human brain tissue by IHC-P.
Immunofluorescence of GluK5 in Human Brain cells using ab67408 at 20 ug/ml.
This product has been referenced in:
- Zhu Z et al. Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation. Sci Rep 6:19069 (2016). Read more (PubMed: 26753906) »
- Funk JA et al. Characterization of Renal Toxicity in Mice Administered the Marine Biotoxin Domoic Acid. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24511141) »