Anti-GluK5 antibody (ab67408)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-GluK5 antibody
See all GluK5 primary antibodies -
Description
Rabbit polyclonal to GluK5 -
Host species
Rabbit -
Specificity
ab67408 does not cross-react with GRIK4. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human GluK5 (C terminal).
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Positive control
- Human brain tissue and brain tissue lysate.
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General notes
This product was previously labelled as GRIK5
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 109 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2901 Human
- Entrez Gene: 14809 Mouse
- Entrez Gene: 24407 Rat
- Omim: 600283 Human
- SwissProt: Q16478 Human
- SwissProt: Q61626 Mouse
- SwissProt: Q63273 Rat
- Unigene: 367799 Human
see all -
Alternative names
- EAA 2 antibody
- EAA2 antibody
- Excitatory amino acid receptor 2 antibody
see all
Images
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Western blot - Anti-GluK5 antibody (ab67408)Lane 1 : Anti-GluK5 antibody (ab67408) at 0.5 µg/ml
Lane 2 : Anti-GluK5 antibody (ab67408) at 1 µg/ml
Lane 3 : Anti-GluK5 antibody (ab67408) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 109 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK5 antibody (ab67408)ab67408 at 2.5µg/ml staining of GluK5 in human brain tissue by IHC-P.
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Immunocytochemistry/ Immunofluorescence - Anti-GluK5 antibody (ab67408)Immunofluorescence of GluK5 in Human Brain cells using ab67408 at 20 ug/ml.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhu Z et al. Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation. Sci Rep 6:19069 (2016). Read more (PubMed: 26753906) »
- Funk JA et al. Characterization of Renal Toxicity in Mice Administered the Marine Biotoxin Domoic Acid. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24511141) »
