Overview

  • Product name

    Anti-GluN2C antibody [EPR19046]
    See all GluN2C primary antibodies
  • Description

    Rabbit monoclonal [EPR19046] to GluN2C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    within Rat GluN2C aa 300-400. The exact sequence is proprietary.
    Database link: Q00961

  • Positive control

    • WB: Mouse and rat cerebellum lysates; Mouse GluN2C fragment recombinant protein.
  • General notes

     

     

    Previously labelled as NMDAR2C. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19046
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Function

      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • Tissue specificity

      Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.
    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.
    • Cellular localization

      Cell membrane. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • GluN2C antibody
      • Glutamate [NMDA] receptor subunit epsilon-3 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody
      • Glutamate receptor ionotropic, NMDA 2C antibody
      • Glutamate receptor, ionotropic, N methyl D aspartate 2C antibody
      • Grin2c antibody
      • N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody
      • N Methyl D Aspartate Receptor Subtype 2C antibody
      • N methyl D aspartate receptor subunit 2C antibody
      • N-methyl D-aspartate receptor subtype 2C antibody
      • NMDAR2C antibody
      • NMDE3_HUMAN antibody
      • NR2C antibody
      • OTTHUMP00000041930 antibody
      see all

    Images

    • All lanes : Anti-GluN2C antibody [EPR19046] (ab192831) at 1/1000 dilution

      Lane 1 : Mouse cerebellum lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 4 : Rat liver lysate
      Lane 5 : Rat cerebellum lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 135 kDa
      Observed band size: 135 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,2,3 and 4: 1 minute; Lane 5: 15 seconds.

    • All lanes : Anti-GluN2C antibody [EPR19046] (ab192831) at 1/1000 dilution

      Lane 1 : Mouse GluN2C fragment recombinant protein
      Lane 2 : Mouse NMDAR2D fragment recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 135 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

      Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house. NMDAR2A and NMDAR2B were not tested, as bands of their size (180 kD) were not detected in tissue lysates.

    References

    ab192831 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab192831.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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