Recombinant Anti-GluN2C antibody [EPR19094] (ab182277)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19094] to GluN2C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-GluN2C antibody [EPR19094]
See all GluN2C primary antibodies -
Description
Rabbit monoclonal [EPR19094] to GluN2C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse GluN2C fragment recombinant protein; Mouse and rat cerebellum lysates; Human fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19094 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2905 Human
- Entrez Gene: 14813 Mouse
- Entrez Gene: 24411 Rat
- Omim: 138254 Human
- SwissProt: Q14957 Human
- SwissProt: Q01098 Mouse
- SwissProt: Q00961 Rat
- Unigene: 436980 Human
see all -
Alternative names
- GluN2C antibody
- Glutamate [NMDA] receptor subunit epsilon-3 antibody
- Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody
see all
Images
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All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/2000 dilution
Lane 1 : Mouse GluN2C fragment recombinant protein
Lane 2 : Mouse NMDAR2D fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane1: 1 second; Lane2: 3 minutes.
Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house.
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All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution
Lane 1 : Rat cerebellum lysate
Lane 2 : Mouse cerebellum lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat liver lysate
Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile is consistent with what has been described in the literature (PMID: 8987814).
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Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab182277 has not yet been referenced specifically in any publications.