Key features and details
- Rabbit polyclonal to GluN2D
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-GluN2D antibody
See all GluN2D primary antibodies
DescriptionRabbit polyclonal to GluN2D
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)
This product was previously labelled as Glutamate receptor ionotropic
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab35448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EB11 antibody
- estrogen receptor binding CpG island antibody
- GluN2D antibody
All lanes : Anti-GluN2D antibody (ab35448) at 1 µg/ml
Lane 1 :
Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
Lane 4 : Brain (Rat) Tissue Lysate, 7 days old
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa
Observed band size: 143 kDa
Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.
ab35448 has been referenced in 1 publication.
- Zhou X et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet 19:3797-805 (2010). WB ; Mouse . PubMed: 20634195