Key features and details
- Rabbit polyclonal to GluN2D
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-GluN2D antibody
See all GluN2D primary antibodies
DescriptionRabbit polyclonal to GluN2D
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)
This product was previously labelled as Glutamate receptor ionotropic
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab35448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EB11 antibody
- estrogen receptor binding CpG island antibody
- GluN2D antibody
All lanes : Anti-GluN2D antibody (ab35448) at 1 µg/ml
Lane 1 :
Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
Lane 4 : Brain (Rat) Tissue Lysate, 7 days old
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa
Observed band size: 143 kDa
Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.
ab35448 has been referenced in 1 publication.
- Zhou X et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet 19:3797-805 (2010). WB ; Mouse . PubMed: 20634195