Overview

  • Product name

  • Description

    Rabbit polyclonal to GLUT10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human GLUT10 aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab138808)

  • Positive control

    • This antibody gave positive in Human Pancreas tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.
  • General notes

    Previously labelled as Glucose Transporter GLUT10. 

Properties

Applications

Our Abpromise guarantee covers the use of ab110528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Facilitative glucose transporter.
  • Tissue specificity

    Widely expressed; highest levels in liver and pancreas.
  • Involvement in disease

    Defects in SLC2A10 are the cause of arterial tortuosity syndrome (ATS) [MIM:208050]. ATS is an autosomal recessive disorder characterized by tortuosity and elongation of major arteries, often resulting in death at young age. Other typical features include aneurysms of large arteries and stenosis of the pulmonary artery, in association with facial features and several connective tissue manifestations such as soft skin and joint laxity. Histopathological findings include fragmentation of elastic fibers in the tunica media of large arteries.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization

    Endomembrane system. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA450473 antibody
    • ATS antibody
    • facilitated glucose transporter member 10 antibody
    • Glucose Transporter GLUT10 antibody
    • Glucose transporter type 10 antibody
    • glut 10 antibody
    • GLUT-10 antibody
    • GLUT10 antibody
    • GTR10_HUMAN antibody
    • MGC126706 antibody
    • slc2a10 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2 member 10 antibody
    • Solute carrier family 2, facilitated glucose transporter member 10 antibody
    see all

Images

  • IHC image of GLUT10 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110528, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-GLUT10 antibody (ab110528) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References

This product has been referenced in:

  • Taggart K  et al. PDK4 Deficiency Induces Intrinsic Apoptosis in Response to Starvation in Fibroblasts from Doberman Pinschers with Dilated Cardiomyopathy. Biores Open Access 6:182-191 (2017). Read more (PubMed: 29285418) »
See 1 Publication for this product

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