• Product name

  • Description

    Rabbit polyclonal to GLUT12
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human GLUT12 aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab113551)

  • Positive control

    • This antibody gave a positive signal in both Human liver membrane and Human liver tissue lysates.



Our Abpromise guarantee covers the use of ab100993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 67 kDa).


  • Function

    Facilitative glucose transporter.
  • Tissue specificity

    Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational

  • Cellular localization

    Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily perinuclear region in the absence of insulin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glucose transporter type 12 antibody
    • GLUT 12 antibody
    • GLUT-12 antibody
    • GLUT12 antibody
    • GLUT8 antibody
    • GTR12_HUMAN antibody
    • SLC2A12 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 12 antibody
    • Solute carrier family 2 facilitated glucose transporter member 12 antibody
    • Solute carrier family 2 member 12 antibody
    • Solute carrier family 2, facilitated glucose transporter member 12 antibody
    see all


  • All lanes : Anti-GLUT12 antibody (ab100993) at 1 µg/ml

    Lane 1 : Human liver tissue lysate (cirrhosis) - membrane extract (ab29881)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    GLUT12 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab100993 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab100993 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Shimamoto S  et al. Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes. Gen Comp Endocrinol 283:113232 (2019). Read more (PubMed: 31356813) »
  • Coudert E  et al. Phylogenesis and Biological Characterization of a New Glucose Transporter in the Chicken (Gallus gallus), GLUT12. PLoS One 10:e0139517 (2015). Read more (PubMed: 26431526) »
See all 2 Publications for this product

Customer reviews and Q&As


Thank you very much for your inquiry.

I can confirm that we do not have any antibodies against GLUT11.

But I am happy to let you know that we have an anti GLUT7 antibody that is tested and guaranteed for IHC-P. It is named after SLC2A7 which is an alternative name for GLUT7:


https://www.abcam.com/index.html?datasheet=122604 (or use the following: https://www.abcam.com/index.html?datasheet=122604).

For GLUT8 and GLUT12 we have antibodies but none that is tested for IHC-P.

If you would like to test this yourself, you would be eligible for our Abtrial program:


I could offer the following antibodies for this program:

GLUT12 : testing discount for ab75441 or ab100993

https://www.abcam.com/index.html?datasheet=75441 (or use the following: https://www.abcam.com/index.html?datasheet=75441).

https://www.abcam.com/index.html?datasheet=100993 (or use the following: https://www.abcam.com/index.html?datasheet=100993).

GLUT8 : testing discount for ab99132

https://www.abcam.com/index.html?datasheet=99132 (or use the following: https://www.abcam.com/index.html?datasheet=99132).

I hope this information is helpful and wish you good luck for your research.

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