Overview

  • Product name

    Anti-GLUT6 antibody
  • Description

    Rabbit polyclonal to GLUT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human GLUT6 aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab119273)

  • Positive control

    • This antibody gave a positive signal in Human Brain tissue lysate.
  • General notes

    Previously labelled as Glucose Transporter GLUT6.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Facilitative glucose transporter; binds cytochalasin B with low affinity.
  • Tissue specificity

    Highly expressed in brain, spleen and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization

    Cell membrane. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • Database links

  • Alternative names

    • A330096C23 antibody
    • F630103L12Rik antibody
    • Glucose Transporter GLUT6 antibody
    • Glucose transporter type 6 antibody
    • Glucose transporter type 9 antibody
    • GLUT-6 antibody
    • GLUT-9 antibody
    • Glut6 antibody
    • Glut9 antibody
    • GTR6_HUMAN antibody
    • HSA011372 antibody
    • RP23-449M10.2 antibody
    • SLC2A6 antibody
    • Solute carrier family 2, facilitated glucose transporter member 6 antibody
    see all

Images

  • Anti-GLUT6 antibody (ab119272) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 8 minutes
  • ab119272 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119272 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab119272 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up