Anti-Glutamate-ammonia ligase antibody (ab243208)
Key features and details
- Rabbit polyclonal to Glutamate-ammonia ligase
- Suitable for: WB
- Reacts with: Arabidopsis thaliana, Corn
- Isotype: IgG
Overview
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Product name
Anti-Glutamate-ammonia ligase antibody -
Description
Rabbit polyclonal to Glutamate-ammonia ligase -
Host species
Rabbit -
Specificity
This antibody recognizes plant Glutamine Synthetase isoproteins including those of Maize, Spinach and Arabidopsis.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana, Corn -
Immunogen
Recombinant full length protein corresponding to Glutamate-ammonia ligase. Maize. (Purified).
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Positive control
- WB: E.coli extracts overexpressing recombinant Maize root type GS isoproteins. Arabidopsis and Maize leaf extract.
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General notes
This product was previously labelled as GLN6
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Filter sterilized. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
Target
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Relevance
Plays a role in the flow of nitrogen into nitrogenous organic compounds. Five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.
Images
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Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)All lanes : Anti-Glutamate-ammonia ligase antibody (ab243208) at 1/1000 dilution
Lane 1 : Arabidopsis leaf extract
Lane 2 : Maize leaf extract
Lysates/proteins at 2 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 39 kDaThis antibody reacts with both leaf and root types of the isoporteins. Maize leaf contains both types. Asterisk indicates the band for chloroplastic GS (46 kDa) that migrates at position of 51 kDa in SDS-PAGE.
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Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)All lanes : Anti-Glutamate-ammonia ligase antibody (ab243208) at 1/1000 dilution
Lane 1 : Negative control without recombinant GS
Lane 2 : GLN6 (GS1-1) expressing extract
Lane 3 : GS1-2 expressing extract
Lane 4 : GS1-3 expressing extract
Lane 5 : GS1-4 expressing extract
Lane 6 : Maize root extract treated with ammonia
Predicted band size: 39 kDaE.coli extracts overexpressing recombinant maize root type GS isoproteins.
The lower band in Lane 6 is ammonia induced GS.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab243208 has been referenced in 2 publications.
- Kimata-Ariga Y & Hase T Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One 9:e108965 (2014). WB . PubMed: 25271437
- Sakakibara H et al. Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem 271:29561-8 (1996). WB . PubMed: 8939884