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Anti-Glutamate-ammonia ligase antibody (ab243208)

  • Datasheet
  • SDS
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Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)
  • Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)

Key features and details

  • Rabbit polyclonal to Glutamate-ammonia ligase
  • Suitable for: WB
  • Reacts with: Arabidopsis thaliana, Corn
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Glutamate-ammonia ligase antibody
  • Description

    Rabbit polyclonal to Glutamate-ammonia ligase
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes plant Glutamine Synthetase isoproteins including those of Maize, Spinach and Arabidopsis.

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Arabidopsis thaliana, Corn
  • Immunogen

    Recombinant full length protein corresponding to Glutamate-ammonia ligase. Maize. (Purified).

  • Positive control

    • WB: E.coli extracts overexpressing recombinant Maize root type GS isoproteins. Arabidopsis and Maize leaf extract.
  • General notes

     This product was previously labelled as GLN6

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.5
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Filter sterilized.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Plays a role in the flow of nitrogen into nitrogenous organic compounds. Five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.

Images

  • Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)
    Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)
    All lanes : Anti-Glutamate-ammonia ligase antibody (ab243208) at 1/1000 dilution

    Lane 1 : Arabidopsis leaf extract
    Lane 2 : Maize leaf extract

    Lysates/proteins at 2 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 39 kDa



    This antibody reacts with both leaf and root types of the isoporteins. Maize leaf contains both types. Asterisk indicates the band for chloroplastic GS (46 kDa) that migrates at position of 51 kDa in SDS-PAGE.

  • Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)
    Western blot - Anti-Glutamate-ammonia ligase antibody (ab243208)
    All lanes : Anti-Glutamate-ammonia ligase antibody (ab243208) at 1/1000 dilution

    Lane 1 : Negative control without recombinant GS
    Lane 2 : GLN6 (GS1-1) expressing extract
    Lane 3 : GS1-2 expressing extract
    Lane 4 : GS1-3 expressing extract
    Lane 5 : GS1-4 expressing extract
    Lane 6 : Maize root extract treated with ammonia

    Predicted band size: 39 kDa



    E.coli extracts overexpressing recombinant maize root type GS isoproteins.

    The lower band in Lane 6 is ammonia induced GS.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab243208? Please let us know so that we can cite the reference in this datasheet.

ab243208 has been referenced in 2 publications.

  • Kimata-Ariga Y & Hase T Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One 9:e108965 (2014). WB . PubMed: 25271437
  • Sakakibara H  et al. Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem 271:29561-8 (1996). WB . PubMed: 8939884

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