For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I'm using the Glutamate Assay kit to detect glutamate level in my Astrocytes cell culture and I observed an increasing levels of glutamate in my culture, without adding any glutamate or even change the cells medium. Astrocytes are cells that uptake the glutamate, so I expected that glutamate levels would decrease instead of increase.
Also, I'm pretty sure that my culture has the majority of Astrocytes cells. So, as the kit doesn't come with any information, I wonder if the kit is capable of detecting other compound besides glutamate, as glutamine, for example.
Attached I'm sending a graph of my results to clarify what I got.
I'll appreciate a quickly reply, if possible.
Asked on Dec 07 2012
Thank you for contacting us.
After doing some research on this subject it seems that astrocytes release glutamate in a Ca2+ dependent manner. It is a very well-known function/property of astrocytes involving transmitter uptake and release.
Here is the wiki page explaining this phenomenon: http://en.wikipedia.org/wiki/Astrocyte
I also add the link to a couple of papers explaining this phenomenon:
The kit (ab83389) is very specific in detecting only glutamate since it utilizes a glutamate specific substrate.
You may need to change certain conditions in the cell media and play around with the incubation times; some glutamate uptake as well should also be seen.
I hope this helps. Please do not hesitate to contact us for further assistance.
Answered on Dec 07 2012