Product nameGlutamate Assay Kit (Fluorometric)
See all Glutamate kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue, Cell Lysate
Assay time0h 30m
Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.
In the glutamate assay protocol, the coupled enzyme system catalyzes the reaction between L-Glutamic acid and NADP+ to produce NADPH, which is specifically recognized by the NADPH sensor and recycled back to NADP+. During the reaction, a red fluorescence product is produced, which in turn can be detected in a fluorescence microplate reader at Ex/Em = 540/590 nm (range Ex/Em = 530 – 570/590 – 600 nm).
This assay can detect as little as 1 µM glutamic acid. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay is robust, and can be easily adapted to automation without separation step as no wash step is required.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min - 2 hr
- analyze with a microplate reader
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 10ml Dilution Buffer 1 x 10ml Enzyme Mix 1 unit Glutamic Acid 1 vial NADP 1 vial
RelevanceGlutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
The concentration of intracellular glutamate in cells, during the exponential phase, grown at different hydrostatic pressures.
Standard curve (30 minutes incubation): mean of duplicates (+/- SD), with background reads subtracted.
Glutamic acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (mean of duplicates, +/- SD).
Glutamic acid measured in cell lysates showing quantity (nmol) per 106 cells (mean of duplicates, +/- SD).
Glutamate measured in human biological fluids (mean of duplicates, +/- SD).
ab138883 has been referenced in 6 publications.
- Agudelo LZ et al. Skeletal muscle PGC-1a1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance. Nat Commun 10:2767 (2019). PubMed: 31235694
- Lim SH et al. Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification. Sci Rep 8:1596 (2018). PubMed: 29371611
- Suina K et al. Epidermal growth factor receptor promotes glioma progression by regulating xCT and GluN2B-containing N-methyl-d-aspartate-sensitive glutamate receptor signaling. Cancer Sci 109:3874-3882 (2018). PubMed: 30298963
- Proust A et al. Contrasting effect of the latency-reversing agents bryostatin-1 and JQ1 on astrocyte-mediated neuroinflammation and brain neutrophil invasion. J Neuroinflammation 14:242 (2017). PubMed: 29228979
- Zhou J et al. Inhibition of LIN28B impairs leukemia cell growth and metabolism in acute myeloid leukemia. J Hematol Oncol 10:138 (2017). PubMed: 28693523
- Amrani A et al. Transcriptomics reveal several gene expression patterns in the piezophile Desulfovibrio hydrothermalis in response to hydrostatic pressure. PLoS One 9:e106831 (2014). Functional Studies . PubMed: 25215865