Overview

  • Product name

    Glutamate Assay Kit (Fluorometric)
    See all Glutamate kits
  • Detection method

    Fluorescent
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue, Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    1 µM
  • Assay time

    0h 30m
  • Product overview

    Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.


    In the glutamate assay protocol, the coupled enzyme system catalyzes the reaction between L-Glutamic acid and NADP+ to produce NADPH, which is specifically recognized by the NADPH sensor and recycled back to NADP+. During the reaction, a red fluorescence product is produced, which in turn can be detected in a fluorescence microplate reader at Ex/Em = 540/590 nm (range Ex/Em = 530 – 570/590 – 600 nm).


    This assay can detect as little as 1 µM glutamic acid. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.


    The assay is robust, and can be easily adapted to automation without separation step as no wash step is required.


    Glutamate assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 30 min - 2 hr
    - analyze with a microplate reader

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    Assay Buffer 1 x 10ml
    Dilution Buffer 1 x 10ml
    Enzyme Mix 1 unit
    Glutamic Acid 1 vial
    NADP 1 vial
  • Research areas

  • Relevance

    Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Images

  • The concentration of intracellular glutamate in cells, during the exponential phase, grown at different hydrostatic pressures.

  • Standard curve (30 minutes incubation): mean of duplicates (+/- SD), with background reads subtracted.

  • Glutamic acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (mean of duplicates, +/- SD).

  • Glutamic acid measured in cell lysates showing quantity (nmol) per 106 cells (mean of duplicates, +/- SD).

  • Glutamate measured in human biological fluids (mean of duplicates, +/- SD).

Protocols

References

This product has been referenced in:

  • Lim SH  et al. Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification. Sci Rep 8:1596 (2018). Read more (PubMed: 29371611) »
  • Suina K  et al. Epidermal growth factor receptor promotes glioma progression by regulating xCT and GluN2B-containing N-methyl-d-aspartate-sensitive glutamate receptor signaling. Cancer Sci 109:3874-3882 (2018). Read more (PubMed: 30298963) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Unfortunately neither of these kits will be able to detect glutamic acid in peptides/proteins. ab83389 only measures free glutamate levels not glutamic acid and ab138883 is based on enzymatic reactions so it is most likely that it will not react with the glutamate in peptide or proteins.

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