For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    glutamate-assay-kit-fluorometric-ab252893.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Amino Acids Glutamate
Share by email

Glutamate Assay Kit (Fluorometric) (ab252893)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Glutamate standard curve
  • Measurement of Glutamate.
  • Measurement of Glutamate amounts.

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Cell culture extracts, Cerebral Spinal Fluid, Serum, Tissue Homogenate

You may also be interested in

ELISA
Product image
Serotonin ELISA Kit (ab133053)
Protein
Product image
Recombinant EN-TEV Protease (mutated S219N) protein (Tagged-His Tag) (ab134877)
Assay
Product image
Glutamine Assay Kit (Colorimetric) (ab197011)

View more associated products

Overview

  • Product name

    Glutamate Assay Kit (Fluorometric)
    See all Glutamate kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Cell culture extracts, Tissue Homogenate, Cerebral Spinal Fluid
  • Assay type

    Quantitative
  • Product overview

    Glutamate Assay Kit (Fluorometric) (ab252893) allows for quantification of Glutamate in biological fluids and tissues. The assay is based on an enzymatic reaction in which a fluorogenic probe is reduced producing a stable signal. The reduced fluorophore produces a strong signal (Ex/Em= 535/587 nm), which is directly proportional to the amount of Glutamate in samples. The assay is simple, reproducible, and can specifically detect as low as 5 pmol of Glutamate in a 100 µL reaction.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Glutamate Assay Buffer 1 x 50ml
    Glutamate Enzyme Mix 1 vial
    Glutamate Standard (0.1 M) 1 x 0.1ml
    Glutamate Substrate Mix 1 vial
    Probe (in DMSO) 1 x 0.4ml
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Amino Acid Assays
  • Relevance

    Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Associated products

    Images

    • Glutamate standard curve
      Glutamate standard curve

      Data from multiple experiments.

    • Measurement of Glutamate.
      Measurement of Glutamate.

      Pooled normal human serum (1:10; 5 µL) and pooled normal human CSF (5 µL; undiluted).

    • Measurement of Glutamate amounts.
      Measurement of Glutamate amounts.

      HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates (0.5 µg protein) and mouse muscle extracts (0.5 µg protein).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab252893? Please let us know so that we can cite the reference in this datasheet.

    ab252893 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab252893.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.