Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified protein, Tissue Homogenate
- Sensitivity: 0.5 µU
Product nameGlutamate Carboxypeptidase II Activity Assay Kit
See all PSMA kits
Sample typeTissue Homogenate, Purified protein
Assay typeEnzyme activity
Assay durationMultiple steps standard assay
This Glutamate Carboxypeptidase II Activity Assay Kit (ab284558) (previously known as PicoProbe Glutamate Carboxypeptidase II Activity Assay Kit K738) is a simple plate-based fluorometric assay for the measurement of Glutamate Carboxypeptidase II (GCPII) activity in Biological Samples. The provided substrate is transaminated in the presence of GCPII producing glutamate. The detection system is based on an enzymatic reaction in which a fluorogenic probe is reduced generating a stable signal. The reduced fluorophore produces a strong signal which is directly proportional to the amount of active GCPII in samples. The assay can detect as low as 0.5 µU of Glutamate Carboxypeptidase II.
This product is manufactured by BioVision, an Abcam company and was previously called K738 PicoProbeTM Glutamate Carboxypeptidase II Activity Assay Kit. K738-100 is the same size as the 100 test size of ab284558.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests GCPII Assay Buffer NM cap 1 x 35ml GCPII Developer Red cap 1 vial GCPII Enzyme Mix Green cap 1 vial GCPII Positive Control Purple cap 1 vial GCPII Substrate Orange cap 1 x 35µl Glutamate Standard (0.1 M) Yellow cap 1 x 0.1ml PicoProbe (in DMSO) Blue cap 1 x 0.4ml
FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- Cell growth inhibiting protein 27
- Cell growth-inhibiting gene 27 protein
ab284558 has not yet been referenced specifically in any publications.