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    glutamate-carboxypeptidase-ii-activity-assay-kit-ab284558.pdf

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Signal Transduction Metabolism Amino Acids
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Glutamate Carboxypeptidase II Activity Assay Kit (ab284558)

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  • SDS
  • Protocol Booklet
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PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
  • PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
  • PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate (12 x 8 well strips)
  • Sample type: Purified protein, Tissue Homogenate
  • Sensitivity: 0.5 µU

Overview

  • Product name

    Glutamate Carboxypeptidase II Activity Assay Kit
    See all PSMA kits
  • Detection method

    Fluorescent
  • Sample type

    Tissue Homogenate, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    0.5 µU
  • Assay duration

    Multiple steps standard assay
  • Product overview

    This Glutamate Carboxypeptidase II Activity Assay Kit (ab284558) (previously known as PicoProbe Glutamate Carboxypeptidase II Activity Assay Kit K738) is a simple plate-based fluorometric assay for the measurement of Glutamate Carboxypeptidase II (GCPII) activity in Biological Samples. The provided substrate is transaminated in the presence of GCPII producing glutamate. The detection system is based on an enzymatic reaction in which a fluorogenic probe is reduced generating a stable signal. The reduced fluorophore produces a strong signal which is directly proportional to the amount of active GCPII in samples. The assay can detect as low as 0.5 µU of Glutamate Carboxypeptidase II.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K738 PicoProbeTM Glutamate Carboxypeptidase II Activity Assay Kit. K738-100 is the same size as the 100 test size of ab284558.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    GCPII Assay Buffer NM cap 1 x 35ml
    GCPII Developer Red cap 1 vial
    GCPII Enzyme Mix Green cap 1 vial
    GCPII Positive Control Purple cap 1 vial
    GCPII Substrate Orange cap 1 x 35µl
    Glutamate Standard (0.1 M) Yellow cap 1 x 0.1ml
    PicoProbe (in DMSO) Blue cap 1 x 0.4ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
  • Function

    Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
    Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • Tissue specificity

    Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.
  • Domain

    The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
  • Post-translational
    modifications

    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm and Cell membrane.
  • Target information above from: UniProt accession Q04609 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Cell growth inhibiting protein 27
    • Cell growth-inhibiting gene 27 protein
    • FGCP
    • Folate hydrolase
    • Folate hydrolase (prostate-specific membrane antigen) 1
    • Folate hydrolase 1
    • Folate hydrolase prostate specific membrane antigen 1
    • FOLH
    • FOLH 1
    • Folh1
    • FOLH1_HUMAN
    • Folylpoly gamma glutamate carboxypeptidase
    • Folylpoly-gamma-glutamate carboxypeptidase
    • GCP 2
    • GCP II
    • GCP2
    • GCPII
    • GIG27
    • Glutamate carboxylase II
    • Glutamate carboxypeptidase 2
    • Glutamate carboxypeptidase II
    • Membrane glutamate carboxypeptidase
    • mGCP
    • N acetylated alpha linked acidic dipeptidase 1
    • N-acetylated-alpha-linked acidic dipeptidase I
    • NAALAD 1
    • NAALAD1
    • NAALAdase
    • NAALADase I
    • Prostate specific membrane antigen
    • Prostate specific membrane antigen variant F
    • Prostate-specific membrane antigen
    • PSM
    • PSMA
    • Pteroylpoly gamma glutamate carboxypeptidase
    • Pteroylpoly-gamma-glutamate carboxypeptidase
    see all

Images

  • PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
    PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit

    Glutamate standard curve, results from multiple experiments

  • PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
    PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit

    Purified glutamate carboxypeptidase II activity and GCPII activity in mouse kidney.

  • PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
    PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit

    Measurement of GCPII activity in mouse kidney extracts (6 µg protein). All assays were performed following kit protocol.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab284558? Please let us know so that we can cite the reference in this datasheet.

ab284558 has not yet been referenced specifically in any publications.

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