Glutamate Carboxypeptidase II Activity Assay Kit (ab284558)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified protein, Tissue Homogenate
- Sensitivity: 0.5 µU
Overview
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Product name
Glutamate Carboxypeptidase II Activity Assay Kit
See all PSMA kits -
Detection method
Fluorescent -
Sample type
Tissue Homogenate, Purified protein -
Assay type
Enzyme activity -
Sensitivity
0.5 µU -
Assay duration
Multiple steps standard assay -
Product overview
This Glutamate Carboxypeptidase II Activity Assay Kit (ab284558) (previously known as PicoProbe Glutamate Carboxypeptidase II Activity Assay Kit K738) is a simple plate-based fluorometric assay for the measurement of Glutamate Carboxypeptidase II (GCPII) activity in Biological Samples. The provided substrate is transaminated in the presence of GCPII producing glutamate. The detection system is based on an enzymatic reaction in which a fluorogenic probe is reduced generating a stable signal. The reduced fluorophore produces a strong signal which is directly proportional to the amount of active GCPII in samples. The assay can detect as low as 0.5 µU of Glutamate Carboxypeptidase II.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K738 PicoProbeTM Glutamate Carboxypeptidase II Activity Assay Kit. K738-100 is the same size as the 100 test size of ab284558.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests GCPII Assay Buffer NM cap 1 x 35ml GCPII Developer Red cap 1 vial GCPII Enzyme Mix Green cap 1 vial GCPII Positive Control Purple cap 1 vial GCPII Substrate Orange cap 1 x 35µl Glutamate Standard (0.1 M) Yellow cap 1 x 0.1ml PicoProbe (in DMSO) Blue cap 1 x 0.4ml -
Research areas
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Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
Tissue specificity
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily. -
Domain
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
Post-translational
modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Alternative names
- Cell growth inhibiting protein 27
- Cell growth-inhibiting gene 27 protein
- FGCP
see all
Images
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PicoProbe™ Glutamate Carboxypeptidase II Activity Assay Kit
Glutamate standard curve, results from multiple experiments
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PicoProbe™ Glutamate Carboxypeptidase II Activity Assay KitPurified glutamate carboxypeptidase II activity and GCPII activity in mouse kidney.
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PicoProbe™ Glutamate Carboxypeptidase II Activity Assay KitMeasurement of GCPII activity in mouse kidney extracts (6 µg protein). All assays were performed following kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284558 has not yet been referenced specifically in any publications.