Overview

  • Product name

    Glutamate Dehydrogenase Activity Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Enzyme activity
  • Sensitivity

    > 0.01 mU/well
  • Product overview

    Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.

  • Platform

    Microplate reader

Properties

Images

  • GDH measured showing activity (mU) per mL of tested sample

  • GDH measured in cell lysates showing activity (mU) per 106 cells tested

  • GDH measured in various samples showing O.D.'s in time course

  • NADH Standard Curve

Protocols

References

This product has been referenced in:

  • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). Functional Studies . Read more (PubMed: 27633083) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

- No special preparation is required for cell culture supernatant. Simply collect the media and use in the assay. It is worth testing different dilutions with GDH assay buffer. I would suggest using 5-50 ul as with serum samples, bringing the final volume up to 50 ul with GDH assay buffer.

- We are not aware of any components of Williams E media interfering with this assay. Phenol red should be fine. We would not recommend going higher than the glutamine concentration of 0.292g/L.

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Answer

Thank you for contacting Abcam regarding ab102527.


We have not tested this kit with lactobacilli, but theoretically it should work. Further protocol optimization may be necessary for best results.


Unfortunately, we do not have samples available. All of our products are guaranteed by our Abpromise to work as stated on the datasheet. Products that do not work can be replaced or refunded when reported within 6 months of purchase.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for your patience.

Unfortunately, the species protein is propriety and I cannot give you this information. However, the lab just confirmed that this kitab102527 Glutamate Dehydrogenase DetectionKitis suitable for sheep serum.

Good luck with your experience. Do not hesitate to contact us again if you need more advise or information.

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Answer

Thank you for contacting us.

Unfortunately, we just have the aforementioned kit you mentioned butnothing regarding the other enzymes.

As the species is not given, I assumed that the test was established using the human protein. I compared the human protein with the sheep protein, and it looks like the homology is too low to give a result. However, I have contacted the lab to get more information regarding the species protein which was used to established this kit and will confirm this as soon as possible.

Regarding the delivery to Japan, please have a look at our website: you can switch the country to Japan in the right upper corner. As we have actually a branch office in Japan, delivery shouldn't be a problem. the current prize for this product is ¥86,000, and the lead time is up to two weeks. You may need to contact one of our subdealers for your region.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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