Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 40 uU/ml
Product nameGlutamate Oxidase Assay Kit (Fluorometric - Near Infrared)
Sample typeCell Lysate
Assay typeEnzyme activity
Sensitivity= 40 uU/ml
Species reactivityReacts with: Mammals, Other species
Glutamate Oxidase Assay Kit (Fluorometric - Near Infrared) (ab138885) provides a quick and an ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to a-ketoglutarate, NH3 and H2O2 by glutamate oxidase. The kit uses our AbRed Indicator substrate which can react with H2O2 when catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product. The signal can be read with either a fluorescence microplate reader at Ex/Em = 530-570 nm/590 -600 nm (optimal Ex/Em = 540 nm/590 nm) or an absorbance microplate reader at 576±5 nm. With this product, we have detected as little as 40 µU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
Glutamate oxidase specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of ketoglutarate, ammonia, and hydrogen peroxide. Glutamate oxidase can be used as an analytic reagent and as a basis for developing biosensors for the determination of L-glutamate, L-glutamine, ammonia, and creatinine. These biosensors can be used in clinical biochemistry for the determination of glutamate-pyruvate transaminase and glutamate-oxalacetate transaminase in biological fluids, which makes the early diagnosis of heart and liver diseases possible.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests AbRed Indicator 1 vial Assay Buffer 1 x 20ml DMSO 1 x 200µl Glutamate Oxidase Standard 1 vial Glutamic Acid 1 x 3.4mg Horseradish Peroxidase 1 vial
ab138885 has not yet been referenced specifically in any publications.