Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
DescriptionRabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)
SpecificityThis antibody is specific for Glutamate Receptor 1 (AMPA subtype).
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Human
Synthetic peptide within Human Glutamate Receptor 1 (AMPA subtype) aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: SH-SY5Y cell lysate. IHC: human brain tissue.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa).|
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
Tissue specificityWidely expressed in brain.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y cell lysate.
Predicted band size: 101 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.
Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32436 has been referenced in 5 publications.
- Taylor BK et al. Opioid receptors inhibit the spinal AMPA receptor Ca2+ permeability that mediates latent pain sensitization. Exp Neurol 314:58-66 (2019). PubMed: 30660616
- Emanuele M et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine 7:191-204 (2016). PubMed: 27322472
- Qiu S et al. GluA1 phosphorylation contributes to postsynaptic amplification of neuropathic pain in the insular cortex. J Neurosci 34:13505-15 (2014). PubMed: 25274827
- Plaza-Zabala A et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. Neurosci Lett 557 Pt B:101-6 (2013). WB ; Rat . PubMed: 24262606
- van Vuurden DG et al. Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment. PLoS One 4:e5953 (2009). IHC-P ; Human . PubMed: 19536293