Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free (ab247259)
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Overview
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Product nameAnti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] - BSA and Azide free
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
DescriptionRabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free
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Host speciesRabbit
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Specificity
This antibody is specific for Glutamate Receptor 1 (AMPA subtype).
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Tested applicationsSuitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Glutamate Receptor 1 (AMPA subtype) aa 1-100 (N terminal). The exact sequence is proprietary.
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General notes
ab247259 is a PBS-only buffer format of ab32436. Please refer to ab32436 for recommended dilutions, protocols, and image data.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Concentration information loading... -
ClonalityMonoclonal
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Clone numberE308
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
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Tissue specificityWidely expressed in brain.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
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Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Omim: 138248 Human
- SwissProt: P42261 Human
- Unigene: 519693 Human
- Unigene: 737709 Human
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Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
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Datasheets and documents
References
ab247259 has not yet been referenced specifically in any publications.
