Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
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Positive control
- WB: Human, Mouse and Rat brain lysates. IP: Mouse brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.94 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5479 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618). For unpurified use at 1/10000 - 1/50000. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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| IP | 1/10 - 1/100. |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-318097-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Rat brain lysates prepared in RIPA lysis method
Lane 4 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Mouse brain lysates prepared in RIPA lysis method
Lane 6 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDa
Observed band size: 102 kDaExposure times:
Lanes 1 and 2: 180 seconds
Lanes 3 and 4: 50 seconds
Lanes 5 and 6: 60 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-122320-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-251243-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Other - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-122319-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479.jpg)
Other - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
Protocols
References (26)
ab109450 has been referenced in 26 publications.
- Seo MK et al. AMPA receptor-mTORC1 signaling activation is required for neuroplastic effects of LY341495 in rat hippocampal neurons. Sci Rep 10:993 (2020). PubMed: 31969673
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987
- Troyano-Rodriguez E et al. PRRT1 regulates basal and plasticity-induced AMPA receptor trafficking. Mol Cell Neurosci 98:155-163 (2019). PubMed: 31216424
- Gong Q et al. Proteomic profiling of the neurons in mice with depressive-like behavior induced by corticosterone and the regulation of berberine: pivotal sites of oxidative phosphorylation. Mol Brain 12:118 (2019). PubMed: 31888678
- Alvarez-Carbonell D et al. Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathog 15:e1008249 (2019). PubMed: 31887215
