Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-318097-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR5479] to Glutamate Receptor 1 (AMPA subtype) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab219618) -
Positive control
- WB: Human, Mouse and Rat brain lysates. IP: Mouse brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.94 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5479 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618).
For unpurified use at 1/10000 - 1/50000. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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| IP | (1) |
1/10 - 1/100.
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| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618). For unpurified use at 1/10000 - 1/50000. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
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IP
1/10 - 1/100. |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Rat brain lysates prepared in RIPA lysis method
Lane 4 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Mouse brain lysates prepared in RIPA lysis method
Lane 6 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDa
Observed band size: 102 kDaExposure times:
Lanes 1 and 2: 180 seconds
Lanes 3 and 4: 50 seconds
Lanes 5 and 6: 60 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-122320-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-251243-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-122319-anti-glutamate-receptor-1-ampa-subtype-antibody-epr5479.jpg)
OI-RD Scanning - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) -
![Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](https://www.abcam.com/ps/products/109/ab109450/Images/ab109450-7-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab109450 has been referenced in 35 publications.
- Wang X et al. Effects of ketogenic diet on cognitive function in pentylenetetrazol-kindled rats. Epilepsy Res 170:106534 (2021). PubMed: 33385944
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Luo Q et al. Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage. Theranostics 11:5986-6005 (2021). PubMed: 33897894
- Esteras N et al. Mitochondrial ROS control neuronal excitability and cell fate in frontotemporal dementia. Alzheimers Dement N/A:N/A (2021). PubMed: 34057756
- Martin EE et al. Interaction and Subcellular Association of PRRT1/SynDIG4 With AMPA Receptors. Front Synaptic Neurosci 13:705664 (2021). PubMed: 34408636