Immunohistochemistry (Frozen sections) abreview for Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Hippocampus)
Specification
Hippocampus
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% BSA in 0.3% Triton-X PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/1000

Additional data

Abcam response
Please note that original customer image was red (detected with Alexa Fluor 555) but when processed, the image colour changed to blue.

Abcam user community

Verified customer

Submitted Feb 10 2014

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