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  1. Link

    glutamate-receptor-1-ampa-subtype-antibody-n3551-n-terminal-ab174785.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Glutamate Receptors
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Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)

  • Datasheet
Reviews (1) Submit a question References (7)

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Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)

Key features and details

  • Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Recombinant Human Glutamate Receptor 1 (AMPA subtype) protein (ab112297)
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Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

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Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description

    Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
  • Host species

    Mouse
  • Specificity

    Does not cross- react with GluR2.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Recombinant fragment within Rat Glutamate Receptor 1 (AMPA subtype) aa 1-400 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. within extracellular domain
    Database link: P19490

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat brain membrane lysate
  • General notes

    The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N355/1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • AMPA / Kainate
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Glutamate Receptor 1 (AMPA subtype) protein (ab112297)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB (1)
1/1000. Predicted molecular weight: 102 kDa.

ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate  using goat anti-mouse HRP conjugayed antibody as the secondary antibody.

Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/1000. Predicted molecular weight: 102 kDa.

ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate  using goat anti-mouse HRP conjugayed antibody as the secondary antibody.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Tissue specificity

    Widely expressed in brain.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Target information above from: UniProt accession P42261 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2890 Human
    • Entrez Gene: 50592 Rat
    • Omim: 138248 Human
    • SwissProt: P42261 Human
    • SwissProt: P19490 Rat
    • Unigene: 519693 Human
    • Unigene: 29971 Rat
    • Alternative names

      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
      Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)

      Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.

       

    • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
      Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
      Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg

      Secondary
      Goat Anti-Mouse HRP at 1/200 dilution

      Predicted band size: 102 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (7)

    Publishing research using ab174785? Please let us know so that we can cite the reference in this datasheet.

    ab174785 has been referenced in 7 publications.

    • Hu J  et al. Constraint-induced movement therapy enhances AMPA receptor-dependent synaptic plasticity in the ipsilateral hemisphere following ischemic stroke. Neural Regen Res 16:319-324 (2021). PubMed: 32859791
    • Hu J  et al. Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus. Brain Res Bull 160:8-23 (2020). PubMed: 32298779
    • Bingor A  et al. Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors. Nat Commun 11:3688 (2020). PubMed: 32703948
    • Notaras M  et al. UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function. Mol Psychiatry 25:3360-3379 (2020). PubMed: 31636381
    • Kwon Y  et al. ßPix heterozygous mice have defects in neuronal morphology and social interaction. Biochem Biophys Res Commun 516:1204-1210 (2019). PubMed: 31296385
    • Szíber Z  et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell 28:285-295 (2017). WB ; Mouse . PubMed: 27852895
    • Arora K  et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). PubMed: 28545069

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Glutamate Receptor 1 (AMPA subtype) antibody [S3551-1] - N-terminal

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Sample
    Mouse Cell lysate - whole cell (hippocampal neuronal culture)
    Specification
    hippocampal neuronal culture
    Blocking step
    Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
    Read More

    MS. Zsófia Szíber

    Verified customer

    Submitted Mar 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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