Overview

  • Product name
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description
    Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
  • Host species
    Mouse
  • Specificity
    Does not cross- react with GluR2.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Cynomolgus monkey
  • Immunogen

    Fusion protein corresponding to Rat Glutamate Receptor 1 (AMPA subtype) aa 1-389 (N terminal). within extracellular domain
    Sequence:

    MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV TGFQLVNYTD TIPARIMQQW RTSDSRDHTR VDWKRPKYTS ALTYDGVKVM AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFV


    Database link: P19490

  • Positive control
    • WB: Rat brain membrane lysate
  • General notes

    The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab174785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 102 kDa.

ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate  using goat anti-mouse HRP conjugayed antibody as the secondary antibody.

Target

  • Function
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Tissue specificity
    Widely expressed in brain.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.

     

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg

    Secondary
    Goat Anti-Mouse HRP at 1/200 dilution

    Predicted band size: 102 kDa

References

This product has been referenced in:
  • Szíber Z  et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell 28:285-295 (2017). WB ; Mouse . Read more (PubMed: 27852895) »
  • Arora K  et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). Read more (PubMed: 28545069) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Mouse Cell lysate - whole cell (hippocampal neuronal culture)
Specification
hippocampal neuronal culture
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C

Ms. Zsófia Szíber

Verified customer

Submitted Mar 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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