Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Key features and details
- Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal -
Host species
Mouse -
Specificity
Does not cross- react with GluR2. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment within Rat Glutamate Receptor 1 (AMPA subtype) aa 1-400 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. within extracellular domain
Database link: P19490 -
Positive control
- WB: Rat brain membrane lysate
-
General notes
The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N355/1 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/1000. Predicted molecular weight: 102 kDa.
ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody. |
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 102 kDa. ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody. |
Target
-
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
-
Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P19490 Rat
- Unigene: 519693 Human
- Unigene: 29971 Rat
-
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.
-
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg
Secondary
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 102 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (7)
ab174785 has been referenced in 7 publications.
- Hu J et al. Constraint-induced movement therapy enhances AMPA receptor-dependent synaptic plasticity in the ipsilateral hemisphere following ischemic stroke. Neural Regen Res 16:319-324 (2021). PubMed: 32859791
- Hu J et al. Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus. Brain Res Bull 160:8-23 (2020). PubMed: 32298779
- Bingor A et al. Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors. Nat Commun 11:3688 (2020). PubMed: 32703948
- Notaras M et al. UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function. Mol Psychiatry 25:3360-3379 (2020). PubMed: 31636381
- Kwon Y et al. ßPix heterozygous mice have defects in neuronal morphology and social interaction. Biochem Biophys Res Commun 516:1204-1210 (2019). PubMed: 31296385
- Szíber Z et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell 28:285-295 (2017). WB ; Mouse . PubMed: 27852895
- Arora K et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). PubMed: 28545069