Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
DescriptionMouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
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Host speciesMouse
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SpecificityDoes not cross- react with GluR2.
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Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human, Cynomolgus monkey
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Immunogen
Fusion protein corresponding to Rat Glutamate Receptor 1 (AMPA subtype) aa 1-389 (N terminal). within extracellular domain
Sequence:MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV TGFQLVNYTD TIPARIMQQW RTSDSRDHTR VDWKRPKYTS ALTYDGVKVM AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFV
Database link: P19490 -
Positive control
- WB: Rat brain membrane lysate
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General notes
The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberN355/1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 102 kDa. ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody. |
Target
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FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
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Tissue specificityWidely expressed in brain.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
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Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)](https://www.abcam.com/ps/products/174/ab174785/Images/ab174785-316908-anti-glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)](https://www.abcam.com/ps/products/174/ab174785/Images/ab174785-309951-anti-glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg
Secondary
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 102 kDa
Datasheets and documents
References
This product has been referenced in:
- Szíber Z et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell 28:285-295 (2017). WB ; Mouse . Read more (PubMed: 27852895) »
- Arora K et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). Read more (PubMed: 28545069) »
