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Fusion protein corresponding to Rat Glutamate Receptor 1 (AMPA subtype) aa 1-389 (N terminal). within extracellular domain
MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV TGFQLVNYTD TIPARIMQQW RTSDSRDHTR VDWKRPKYTS ALTYDGVKVM AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFV
The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.
Our Abpromise guarantee covers the use of ab174785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 102 kDa.
ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.
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