Recombinant
RabMAb

Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)

Rabbit recombinant monoclonal Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]. Validated in WB, IP, Dot and tested in Mouse, Rat, Human. Cited in 7 publication(s).

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description

    Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)
  • Host species

    Rabbit
  • Specificity

    ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831.
  • Tested applications

    Suitable for: WB, IP, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 831 of Human Glutamate Receptor 1 (AMPA subtype).

  • Positive control

    • Rat brain lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).
IP 1/10 - 1/100.
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • Tissue specificity

      Widely expressed in brain.
    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • Post-translational
      modifications

      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • Database links

    • Alternative names

      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Images

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

      Lane 1 : Mouse hippocampus whole cell lysates
      Lane 2 : Mouse hippocampus whole cell lysates, the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 102 kDa
      Observed band size: 106 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • Dot blot analysis of Glutamate Receptor 1 (AMPA subtype)  with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.

      Lane 1:  Glutamate Receptor 1 (AMPA subtype)   (pS831) phospho peptide
      Lane 2:  Glutamate Receptor 1 (AMPA subtype)   non-phospho peptide

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

      Lane 1 : Human cerebellum whole cell lysates
      Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 102 kDa
      Observed band size: 106 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

      Lane 1 : Rat brain lysates, untreated
      Lane 2 : Rat brain lysates treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 106 kDa why is the actual band size different from the predicted?



      The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.

    References

    This product has been referenced in:

    • Li N  et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). Read more (PubMed: 30837867) »
    • Zhang F  et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). Read more (PubMed: 30651859) »
    See all 9 Publications for this product

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