Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278142-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831) -
Host species
Rabbit -
Specificity
ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831. -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat brain lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1887 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).
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| Dot blot |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa). |
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Dot blot
Use at an assay dependent concentration. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Mouse hippocampus whole cell lysates
Lane 2 : Mouse hippocampus whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
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![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278140-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-dot-blot.jpg)
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS831) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278141-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-122351-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Rat brain lysates, untreated
Lane 2 : Rat brain lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation. -
![Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-5-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab109464 has been referenced in 21 publications.
- Elhussiny MEA et al. Modulation by chronic stress and ketamine of ionotropic AMPA/NMDA and metabotropic glutamate receptors in the rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry 104:110033 (2021). PubMed: 32640261
- Bessières B et al. Early life experiences selectively mature learning and memory abilities. Nat Commun 11:628 (2020). PubMed: 32005863
- Zhang Y et al. Monocular Deprivation Affects Visual Cortex Plasticity Through cPKC?-Modulated GluR1 Phosphorylation in Mice. Invest Ophthalmol Vis Sci 61:44 (2020). PubMed: 32343785
- Gu Y et al. Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII. Neural Plast 2020:8861994 (2020). PubMed: 33488694
- Zhu YB et al. Activation of CaMKII and GluR1 by the PSD-95-GluN2B Coupling-Dependent Phosphorylation of GluN2B in the Spinal Cord in a Rat Model of Type-2 Diabetic Neuropathic Pain. J Neuropathol Exp Neurol 79:800-808 (2020). PubMed: 32386416