RecombinantRabMAb

Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)

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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
  • Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
  • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)

  • Host species

    Rabbit

  • Specificity

    ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831.

  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Rat brain lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).
Dot blot
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).
Dot blot
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC or IHC-P.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

  • Tissue specificity

    Widely expressed in brain.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Images

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

    Lane 1 : Mouse hippocampus whole cell lysates
    Lane 2 : Mouse hippocampus whole cell lysates, the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa
    Observed band size: 106 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)

    Dot blot analysis of Glutamate Receptor 1 (AMPA subtype)  with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.

    Lane 1:  Glutamate Receptor 1 (AMPA subtype)   (pS831) phospho peptide
    Lane 2:  Glutamate Receptor 1 (AMPA subtype)   non-phospho peptide

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

    Lane 1 : Human cerebellum whole cell lysates
    Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa
    Observed band size: 106 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution

    Lane 1 : Rat brain lysates, untreated
    Lane 2 : Rat brain lysates treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa
    Observed band size: 106 kDa why is the actual band size different from the predicted?



    The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.
  • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)

References (17)

Publishing research using ab109464? Please let us know so that we can cite the reference in this datasheet.

ab109464 has been referenced in 17 publications.

  • Bessières B  et al. Early life experiences selectively mature learning and memory abilities. Nat Commun 11:628 (2020). PubMed: 32005863
  • Zhang Y  et al. Monocular Deprivation Affects Visual Cortex Plasticity Through cPKC?-Modulated GluR1 Phosphorylation in Mice. Invest Ophthalmol Vis Sci 61:44 (2020). PubMed: 32343785
  • Gu Y  et al. Electroacupuncture Attenuates CFA-Induced Inflammatory Pain by Regulating CaMKII. Neural Plast 2020:8861994 (2020). PubMed: 33488694
  • Li N  et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867
  • Zhang F  et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). PubMed: 30651859
View all Publications for this product

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