Recombinant Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S831) -
Host species
Rabbit -
Specificity
ab109464 only detects Glutamate Receptor 1 phosphorylated at Serine 831. -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 831 of Human Glutamate Receptor 1 (AMPA subtype).
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Positive control
- Rat brain lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1887 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa). | |
| Dot blot | Use at an assay dependent concentration. |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278142-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Mouse hippocampus whole cell lysates
Lane 2 : Mouse hippocampus whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
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![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278140-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-dot-blot.jpg)
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS831) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-278141-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)](https://www.abcam.com/ps/products/109/ab109464/Images/ab109464-122351-anti-glutamate-receptor-1-ampa-subtype-phospho-s831-antibody-epr1887-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Rat brain lysates, untreated
Lane 2 : Rat brain lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.
References (13)
ab109464 has been referenced in 13 publications.
- Bessières B et al. Early life experiences selectively mature learning and memory abilities. Nat Commun 11:628 (2020). PubMed: 32005863
- Li N et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867
- Zhang F et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). PubMed: 30651859
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987
- Kim JE et al. Blockade of AMPA Receptor Regulates Mitochondrial Dynamics by Modulating ERK1/2 and PP1/PP2A-Mediated DRP1-S616 Phosphorylations in the Normal Rat Hippocampus. Front Cell Neurosci 13:179 (2019). PubMed: 31118889
