Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody (ab3901)
Key features and details
- Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype) (phospho S845) -
Host species
Rabbit -
Specificity
ab3901 is specific for 100 kDa GluR1 phosphorylated at Ser845. Immunolabeling is blocked by the phosphopeptide but not by the dephosphopeptide. -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphoSer845 of the GluR1 subunit of the AMPA subtype of glutamate receptor.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab3901 has been referenced in 6 publications.
- Bonini D et al. Acute Footshock Stress Induces Time-Dependent Modifications of AMPA/NMDA Protein Expression and AMPA Phosphorylation. Neural Plast 2016:7267865 (2016). WB . PubMed: 26966584
- Castilho AF et al. Diabetes causes transient changes in the composition and phosphorylation of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and interaction with auxiliary proteins in the rat retina. Mol Vis 20:894-907 (2014). WB ; Rat . PubMed: 24966661
- Hinard V et al. Key electrophysiological, molecular, and metabolic signatures of sleep and wakefulness revealed in primary cortical cultures. J Neurosci 32:12506-17 (2012). WB ; Mouse . PubMed: 22956841
- Goel A et al. Phosphorylation of AMPA receptors is required for sensory deprivation-induced homeostatic synaptic plasticity. PLoS One 6:e18264 (2011). WB ; Mouse . PubMed: 21483826
- Wang D et al. Amyloid {beta} Peptide-(1-42) Induces Internalization and Degradation of {beta}2 Adrenergic Receptors in Prefrontal Cortical Neurons. J Biol Chem 286:31852-63 (2011). WB . PubMed: 21757762
- Wang D et al. Binding of amyloid beta peptide to beta2 adrenergic receptor induces PKA-dependent AMPA receptor hyperactivity. FASEB J 24:3511-21 (2010). WB ; Rat . PubMed: 20395454
Customer reviews and Q&As
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