Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)
- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
DescriptionRabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
-
Host speciesRabbit
-
Specificityab76321 only detects Glutamate Receptor 1 (AMPA subtype) phosphorylated on Serine 845.
-
Tested applicationsSuitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).
-
Positive control
- WB: Human fetal, rat and mouse brain tissue lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR2148
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab76321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 102 kDa. For unpurified use at 1/1000 - 1/10000. |
|
| Dot blot | 1/1000. |
Target
-
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
-
Tissue specificityWidely expressed in brain.
-
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
-
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
-
Database links
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
Alternative names
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
Images
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-278586-ab76321-WB-on-mouse.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution
Lane 1 : Mouse hippocampus whole cell lysates
Lane 2 : Mouse hippocampus whole cell lysates. The membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutesBlocked in 5% NFDM/TBST
-
![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-278584-ab76321-Dot.jpg)
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) labelling Glutamate Receptor 1 (AMPA subtype) (phospho S845) with ab76321 at 1/1000 in 5% NFDM/TBST. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Exposed for 3 minutes.
Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS845) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-278585-ab76321-WB-on-human.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates. The membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutesBlocked with 5% NFDM/TBST
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-2-ab76321WB1.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1000 cells (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-3-ab76321WB2.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)
Lane 1 : Mouse brain tissue lysate, untreated
Lane 2 : Mouse brain tissue lysate, treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-4-ab76321WB3.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)
Lane 1 : Rat brain tissue lysate, untreated
Lane 2 : Rat brain tissue lysate, treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaBlocking buffer: 5% NFDM/TBST.
Diluting buffer: 1%BSA /TBST.
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)](https://www.abcam.com/ps/products/76/ab76321/Images/ab76321-1.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (unpurified)
Lane 1 : Rat brain tissue lysate, untreated
Lane 2 : Rat brain tissue lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Sutton LP et al. Orphan receptor GPR158 controls stress-induced depression. Elife 7:N/A (2018). Read more (PubMed: 29419376) »
- Penn AC et al. Hippocampal LTP and contextual learning require surface diffusion of AMPA receptors. Nature 549:384-388 (2017). WB ; Rat . Read more (PubMed: 28902836) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"