Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM296] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [RM296]
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
DescriptionRabbit monoclonal [RM296] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).
Database link: P42261
- IHC-P: Human brain tissue. WB: Mouse brain tissue lysate (un-treated).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/200.|
|IHC-P||1/100 - 1/200.|
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
Tissue specificityWidely expressed in brain.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [RM296] (ab222761) at 1/200 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate treated with lambda protein phosphatase
Paraffin embedded human brain tissue stained for Glutamate Receptor 1 (AMPA subtype) (phospho S845) with ab222761 at a 1/200 dilution in Immunohistochemical analysis.
ab222761 has not yet been referenced specifically in any publications.