Western blot abreview for Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (primary cortical culture)
Loading amount
10 µg
Specification
primary cortical culture
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%

Secondary antibody

Name
Non-Abcam antibody was used: jackson
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 130 kDa

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Submitted Dec 23 2008

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