Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Antigen retrieval step
None
Permeabilization
No

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
I used this antibody on sections of rat brain (30 um) in free floating. The antibody produced some staining in cytoplasmic neuronal inclusions. The picture shows the staining obtained using the objective X40 at the level of the cerebral cortex.
The tissues were perfusion fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Abcam response
Many thanks for your feedback on ab12108 (Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody) on rat brain sections on free floating sections using direct immunofluorescence.

Dr. Sophie Pezet

Verified customer

Submitted May 10 2010

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