Recombinant
RabMAb

Recombinant Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

Submit a review Q&A (3)References (11)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y]
    See all Glutamate receptor 3/GluA3 primary antibodies

  • Description

    Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
    Database link: P42263

  • Positive control

    • WB: Human brain tissue lysate.

  • General notes

    Previously labelled as Glutamate receptor 3. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab40845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

    • Involvement in disease

      Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia.

    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

    • Post-translational
      modifications

      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AMPA 3 antibody
      • AMPA selective glutamate receptor 3 antibody
      • AMPA-selective glutamate receptor 3 antibody
      • dJ1171F9.1 antibody
      • GluA3 antibody
      • GLUK3 antibody
      • GluR 3 antibody
      • GLUR C antibody
      • GLUR K3 antibody
      • GluR-3 antibody
      • GluR-C antibody
      • GluR-K3 antibody
      • GLUR3 antibody
      • GLURC antibody
      • Glutamate ionotropic receptor AMPA type subunit 3 antibody
      • Glutamate receptor 3 antibody
      • Glutamate receptor C antibody
      • Glutamate receptor ionotrophic AMPA 3 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor subunit 3 antibody
      • Glutamate receptor, ionotropic, AMPA 3 antibody
      • GRIA 3 antibody
      • Gria3 antibody
      • GRIA3_HUMAN antibody
      • Ionotrophic Glutamate Receptor antibody
      • MRX94 antibody
      see all

    Images

    • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution (purified) + Human hippocampus lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 99 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      All lanes : Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution (purified)

      Lane 1 : Mouse hippocampus lysates
      Lane 2 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 99 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
      Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + human brain lysate at 10 µg

      Predicted band size: 99 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (11)

    Publishing research using ab40845? Please let us know so that we can cite the reference in this datasheet.

    ab40845 has been referenced in 11 publications.

    • Rogers EJ  et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). PubMed: 30842726
    • Mao Q  et al. Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF-ß1/Smad/miR-192 signaling pathway. Exp Ther Med 18:3053-3061 (2019). PubMed: 31572545
    • Zhang H  et al. TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. J Clin Invest 129:3864-3876 (2019). PubMed: 31424425
    • Yu X  et al. Endophilin A1 mediates seizure activity via regulation of AMPARs in a PTZ-kindled epileptic mouse model. Exp Neurol 304:41-57 (2018). WB . PubMed: 29481784
    • Simhal AK  et al. A Computational Synaptic Antibody Characterization Tool for Array Tomography. Front Neuroanat 12:51 (2018). PubMed: 30065633
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    The datasheet is confusing because its difficult to determine if the antibody will specifically detect metaboropic or ionotropic glutamate receptor 3.

    Read More
    Answer

    Thank you for your enquiry.

    I have found that several years ago ab40845 was incorrectly listed as an antibody to metabotropic glutamate receptor 3. However it was then correct to list as ionotropic receptor. Unfortunately some indications from the old metabotropic datasheet survived, including those 2 citations and some alternative names.

    So I can confirm ab40845 is specific for the ionotropic receptor. Also, the immunogen sequence is from amino acid 296 - 314 of the human protein.

    I will contact our IT department to have the metabotropic related items removed from this datasheet to make things more clear.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question

    Hi I would like to know if this antibody is selective for mGulR3 or if it cross reacts with mGluR2? Is it possible to have the peptide sequence so that I can check if this antibody is likely to cross react?

    Read More
    Answer

    I'm afraid we are unable to provide the peptide sequence, however we have performed a sequence alignment of the immunogen with the mGluR2 protein and can confirm that ab40845 is not predicted to cross react with mGluR2 due to very low homology. Please do not hesitate to contact us if you require further information.

    Read More

    Question

    Hello, I am wondering if you might run the epitope homology of this antibody to the X. tropicalis peptide sequence to see if this antibody might work in our system. I hope to use this antibody in a western blot application. Thanks. LFSKAVLLSSSDHNFVRKEIKFEGDLVLGGLFPINEKGGGMDECGRINEDRGIQRLEAML FAIDQINRDSSLLPGVKLGVHILDTCSRDTYALEQSLEFVRASLTKVDEAEYMCPDGSYA IQENSPLPIAGVIGGSYSSVSIQVANLLRLFHIPQISYASTSAKLSDKSRYDYFARTVPP DFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFEHEARLRNICIATSEKQTFFNKN NSAQYVLHLFCKNLISVIDKVGVGQNVSNNTDSIVKFDAYGDGIGLYNVFNFQYHNGKYS YLKIGQWAETLSLDVDIIQWSKSLVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEKYEY LADEFTCIDCGEGRWPTADLTSCFDLPEDYIKWEDAWAIGPVTIACLGFVCTCLVGGVFI KNNSTPLVKASGRELCYILLIGVFMCYCMTFFFIAKPSPVICTLRRLGLGTSFVVCYSAL LTKTNRIARIFSGVKDGAQRPKFISPKSQVVICLSLISVQIVVVSVWLMLEFPGTRRYTL PEKRETVILKCNVKDSSMLISLTYDVVLVILCTVYAFKTRKCPENFNEAKFIGFTMYTTC IIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKN

    Read More
    Answer

    Thank you for your enquiry. I did a sequence alignment between this antibody's immunogen to the sequence provided. Unfortunately, the alignment result showed poor amino acids sequence homology (less than 30% homology). I hope this information will be useful. Should you require any further information, please do not hesitate to contact me.

    Read More

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