Recombinant Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free
See all Glutamate receptor 3/GluA3 primary antibodies -
Description
Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
Database link: P42263 -
Positive control
- WB: Human brain lysate.
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General notes
ab232430 is the carrier-free version of ab40845 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232430 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Glutamate receptor 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP813Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab232430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 101 kDa). |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Involvement in disease
Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2892 Human
- Entrez Gene: 53623 Mouse
- Entrez Gene: 29628 Rat
- Omim: 305915 Human
- SwissProt: P42263 Human
- SwissProt: Q9Z2W9 Mouse
- SwissProt: P19492 Rat
- Unigene: 377070 Human
see all -
Alternative names
- AMPA 3 antibody
- AMPA selective glutamate receptor 3 antibody
- AMPA-selective glutamate receptor 3 antibody
see all
Images
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![Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)](https://www.abcam.com/ps/products/232/ab232430/Images/ab232430-308972-anti-glutamate-receptor-3-glua3-antibody-ep813y-western-blot.jpg)
Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + Human brain lysate at 10 µg
Predicted band size: 101 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40845).
Datasheets and documents
References
ab232430 has not yet been referenced specifically in any publications.
