RecombinantRabMAb

Recombinant Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)

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Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)
  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free
    See all Glutamate receptor 3/GluA3 primary antibodies

  • Description

    Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain lysate.

  • General notes

    ab232430 is the carrier-free version of ab40845 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232430 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Glutamate receptor 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 101 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 101 kDa).

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

  • Involvement in disease

    Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AMPA 3 antibody
    • AMPA selective glutamate receptor 3 antibody
    • AMPA-selective glutamate receptor 3 antibody
    • dJ1171F9.1 antibody
    • GluA3 antibody
    • GLUK3 antibody
    • GluR 3 antibody
    • GLUR C antibody
    • GLUR K3 antibody
    • GluR-3 antibody
    • GluR-C antibody
    • GluR-K3 antibody
    • GLUR3 antibody
    • GLURC antibody
    • Glutamate ionotropic receptor AMPA type subunit 3 antibody
    • Glutamate receptor 3 antibody
    • Glutamate receptor C antibody
    • Glutamate receptor ionotrophic AMPA 3 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor subunit 3 antibody
    • Glutamate receptor, ionotropic, AMPA 3 antibody
    • GRIA 3 antibody
    • Gria3 antibody
    • GRIA3_HUMAN antibody
    • Ionotrophic Glutamate Receptor antibody
    • MRX94 antibody
    see all

Images

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + Human brain lysate at 10 µg

    Predicted band size: 101 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40845).

  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab232430? Please let us know so that we can cite the reference in this datasheet.

ab232430 has not yet been referenced specifically in any publications.

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