Mouse, Rat Predicted to work with:
Synthetic peptide corresponding to Human Glutamate receptor ionotropic, NMDA 2D aa 1300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. Database link: O15399 (Peptide available as ab35447)
This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Western blot - Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448)
All lanes : Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448) at 1 µg/ml
Lane 1 :Mouse brain tissue lysate - total protein (0 days) (ab7188) Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old Lane 4 : Brain (Rat) Tissue Lysate, 7 days old
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa Observed band size: 143 kDa Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.
Zhou X et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet19:3797-805 (2010).
Read more (PubMed: 20634195) »