Product nameAnti-Glutamate receptor ionotropic, NMDA 2D antibody
See all Glutamate receptor ionotropic, NMDA 2D primary antibodies
DescriptionRabbit polyclonal to Glutamate receptor ionotropic, NMDA 2D
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab35448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).Can be blocked with Rat Glutamate receptor ionotropic or NMDA 2D peptide (ab35447).|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EB11 antibody
- estrogen receptor binding CpG island antibody
- GluN2D antibody
All lanes : Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448) at 1 µg/ml
Lane 1 :
Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
Lane 4 : Brain (Rat) Tissue Lysate, 7 days old
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 143 kDa
Observed band size: 143 kDa
Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.