Overview

  • Product name

  • Description

    Rabbit polyclonal to Glutaminase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Glutaminase aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156175)

  • Positive control

    • This antibody gave a positive signal in both Mouse Cortex and Mouse Brain. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab131554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 73 kDa).

Target

  • Function

    Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.
  • Tissue specificity

    KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle.
  • Sequence similarities

    Belongs to the glutaminase family.
    Contains 1 ANK repeat.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAD20 antibody
    • DKFZp686O15119 antibody
    • FLJ10358 antibody
    • GAC antibody
    • GAM antibody
    • GLS antibody
    • GLS1 antibody
    • GLSK_HUMAN antibody
    • Glutaminase C antibody
    • Glutaminase kidney isoform antibody
    • Glutaminase phosphate activated antibody
    • K-glutaminase antibody
    • KGA antibody
    • KIAA0838 antibody
    • L-glutamine amidohydrolase antibody
    • mitochondrial antibody
    see all

Images

  • ICC/IF image of ab131554 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131554 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Glutaminase antibody (ab131554) at 1 µg/ml

    Lane 1 : Mouse Cortex Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 71 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 39 kDa, 92 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    The band observed at 71 kDa could potentially be a cleaved form of Glutaminase due to the presence of a 16 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131554 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab131554 has not yet been referenced specifically in any publications.

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