Immunohistochemistry (Frozen sections) abreview for Anti-Glutaminase antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain section - cortical neurons)
Specification
Brain section - cortical neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Other product details

Dilution
1/80
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% NGS 0.1% Triton X in PBS

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit Cy3 111-165-144 (Jackson Labs)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/400

Additional data

Additional Notes
4% paraformaldehyde used for fixation
normal goat serum used for blocking
counterstained with DAPI to label cell nuclei

Dr. Richard Fairless

Verified customer

Submitted Mar 18 2013

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