Western blot abreview for Anti-Glutaminase antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (NCM460 colon cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
NCM460 colon cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1%
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 60, 65 kDa

Additional data

Additional Notes
Figure legend: Western blotting analysis of total protein fractions of control (lane #1), PD0332991-treated (lane #2) and serum-deprived (lane #3) NCM460 cells. The two isoforms of GLS1 were observed at 65 KDa (KGA isoform) and 60 KDa (GAC isoform).
PubMed ID
28978620: View

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Submitted Oct 02 2018

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